Elastin Monoclonal Antibody AE00168

$520,00

Selective and specific mouse monoclonal antibody clone ELN/1981 to human Elastin

See all our Elastin antibodies

Official Symbol: ELN

GeneID: 2006

Swiss-Prot: P15502

Description

Elastin

All names and symbols: Elastin; Tropoelastin; ELN; ADCL1; SVAS; WBS; WS

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa



Confirmed species reactivityHuman
Confirmed applicationsIHC, PA
Aeonian Rating®80
RRIDAB_2750955



Immunohistochemistry (IHC):

AE00168 to Elastin was successfully used to stain connective tissue in the submucosa of human small intestine sections. Recommended concentration: 1-3ug/ml

IHC of human small intestine stained with Elastin Mouse Monoclonal Antibody AE00168
Human small intestine with AE00168

Formaldehyde-fixed, paraffin-embedded human small intestine stained with Elastin Mouse Monoclonal Antibody AE00168 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00168 to Elastin were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00168 on protein array
AE00168 on HuProt(TM) array

Cross-reactivity assessment of Elastin Mouse Monoclonal Antibody AE00168 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified Elastin Mouse Monoclonal Antibody AE00168. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00168
AE00168 Coomassie bands

Integrity of the purified antibody AE00168 under non-reduced and reduced conditions, showing intact IgG at around 140kDa (NR) and intact heavy and light chains at 45kDa and 26kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant full length human Elastin protein

Epitope

Unknown

Cellular localization

extracellular space, secreted

Positive Controls

A549, intestine, kidney, liver, lung, U2197

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