Ki-67 Monoclonal Antibody AE00190

520,00

Selective and specific mouse monoclonal antibody clone MKI67/2462 to human Ki-67

Official Symbol: MKI67

GeneID: 4288

Swiss-Prot: P46013

Description

Ki-67

All names and symbols: Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; Marker of proliferation Ki-67; Proliferation marker protein Ki-67; KIA; MIB-; MIB-1; MKI67; PPP1R105

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2b, kappa



Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA, WB
Aeonian Rating®80
RRIDAB_2752235



Immunocytochemistry (ICC):

AE00190 to Ki-67 was successfully used to stain nucleoli in cell line HeLa. Recommended concentration: 1-3ug/ml

ICC of HeLa stained with Ki-67 Mouse Monoclonal Antibody AE00190
HeLa with AE00190

Confocal microscopy of HeLa stained with Ki-67 Mouse Monoclonal Antibody AE00190 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and Phalloidin (red) for membrane staining.

Immunohistochemistry (IHC):

AE00190 to Ki-67 was successfully used to stain proliferating lymphocytes in human tonsil sections and of active basal layer cells of human epidermis sections. Recommended concentration: 1-3ug/ml

IHC of human tonsil stained with Ki-67 Mouse Monoclonal Antibody AE00190
Human tonsil with AE00190

Formaldehyde-fixed, paraffin-embedded human tonsil stained with Ki-67 Mouse Monoclonal Antibody AE00190 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human skin stained with Ki-67 Mouse Monoclonal Antibody AE00190
Human skin with AE00190

Formaldehyde-fixed, paraffin-embedded human skin stained with Ki-67 Mouse Monoclonal Antibody AE00190 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00190 to Ki-67 was successfully used to stain approx. 200kDa bands in lysates of cell line Raji and Ramos. Recommended concentration: 0.3-1ug/ml

WB of Raji and Ramos stained with CD22 Mouse Monoclonal Antibody AE00190
Raji and Ramos with AE00190

Western Blot of a Raji and Ramos lysates (30ug) stained with Ki-67 Mouse Monoclonal Antibody AE00190 at 0.3-0.6ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00190 to Ki-67 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00190 on protein array
AE00190 on HuProt(TM) array

Cross-reactivity assessment of Ki-67 Mouse Monoclonal Antibody AE00190 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified Ki-67 Mouse Monoclonal Antibody AE00190. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00190
AE00190 Coomassie bands

Integrity of the purified antibody AE00190 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 55kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 2293-2478 of human Ki-67 protein (exact sequence is proprietary)

Epitope

Within aa 2293-2478 region

Cellular localization

nucleolus

Positive Controls

HeLa, lymph node, proliferating cells, Raji, Ramos, skin, tonsil

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