BOB.1 Monoclonal Antibody AE00200

$520,00

Selective and specific mouse monoclonal antibody clone BOB1/2424 to human BOB.1

Official Symbol: POU2AF1

GeneID: 5450

Swiss-Prot: Q16633

Description

BOB.1

All names and symbols: B-cell-specific coactivator OBF-1; POU class 2 associating factor 1; POU domain class 2-associating factor 1; OCT-binding factor 1; BOB-1; BOB1; BOB.1; OBF-1; OBF1; OCAB; OCA-B; POU2AF1

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2b, kappa



Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA, WB
Aeonian Rating®80
RRIDAB_2813754



Immunocytochemistry (ICC):

AE00200 to BOB.1 was successfully used to stain nuclei in cell line Raji. Recommended concentration: 1-3ug/ml

ICC of Raji stained with BOB.1 Mouse Monoclonal Antibody AE00200
Raji with AE00200

Confocal microscopy of Raji stained with BOB.1 Mouse Monoclonal Antibody AE00200 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.

Immunohistochemistry (IHC):

AE00200 to BOB.1 was successfully used to stain Germinal Centre B-cells perinuclear in human spleen sections. Recommended concentration: 1-3ug/ml

IHC of human spleen stained with BOB.1 Mouse Monoclonal Antibody AE00200
Human spleen with AE00200

Formaldehyde-fixed, paraffin-embedded human spleen stained with BOB.1 Mouse Monoclonal Antibody AE00200 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00200 to BOB.1 was successfully used to stain an approx. 37kDa band in lysates of cell line Raji. Recommended concentration: 0.5-1.5ug/ml

WB of Raji stained with BOB.1 Mouse Monoclonal Antibody AE00200
Raji with AE00200

Western Blot of Raji lysate (30ug) stained with BOB.1 Mouse Monoclonal Antibody AE00200 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00200 to BOB.1 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00200 on protein array
AE00200 on HuProt(TM) array

Cross-reactivity assessment of BOB.1 Mouse Monoclonal Antibody AE00200 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified BOB.1 Mouse Monoclonal Antibody AE00200. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00200
AE00200 Coomassie bands

Integrity of the purified antibody AE00200 under non-reduced and reduced conditions, showing intact IgG at around 160kDa (NR) and intact heavy and light chains at 60kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 148-255 of human BOB.1 protein (exact sequence is proprietary)

Epitope

Within aa 148-255 region

Cellular localization

nucleus

Positive Controls

Raji, spleen

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