BOB.1 Monoclonal Antibody AE00200

Confocal microscopy of Raji stained with BOB.1 Mouse Monoclonal Antibody AE00200 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.

Formaldehyde-fixed, paraffin-embedded human spleen stained with BOB.1 Mouse Monoclonal Antibody AE00200 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot of Raji lysate (30ug) stained with BOB.1 Mouse Monoclonal Antibody AE00200 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Cross-reactivity assessment of BOB.1 Mouse Monoclonal Antibody AE00200 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Integrity of the purified antibody AE00200 under non-reduced and reduced conditions, showing intact IgG at around 160kDa (NR) and intact heavy and light chains at 60kDa and 25kDa resp. (R)