GLUT-1 Monoclonal Antibody AE00202

$520,00

Selective and specific mouse monoclonal antibody clone GLUT1/2476 to human GLUT-1

Official Symbol: SLC2A1

GeneID: 6513

Swiss-Prot: P11166

Description

GLUT-1

All names and symbols: solute carrier family 2 member 1; solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; HepG2 glucose transporter; GLUT-1; GLUT1; CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT1DS; HTLVR; PED; SDCHCN; SLC2A1

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2b, kappa



Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA
Aeonian Rating®82
RRIDAB_2813756

Immunocytochemistry (ICC):

AE00202 to GLUT-1 was successfully used to stain plasma membranes in cell line MCF7. Recommended concentration: 1-3ug/ml

ICC of MCF7 stained with GLUT-1 Mouse Monoclonal Antibody AE00202
MCF7 with AE00202

Confocal microscopy of MCF7 stained with GLUT-1 Mouse Monoclonal Antibody AE00202 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.

Immunohistochemistry (IHC):

AE00202 to GLUT-1 was successfully used to stain peripheral human breast carcinoma sections and to stain smooth muscle cells in human bladder sections. Recommended concentration: 1-3ug/ml

IHC of human breast carcinoma stained with GLUT-1 Mouse Monoclonal Antibody AE00202
Human breast carcinoma with AE00202

Formaldehyde-fixed, paraffin-embedded human breast carcinoma stained with GLUT-1 Mouse Monoclonal Antibody AE00202 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human bladder stained with GLUT-1 Mouse Monoclonal Antibody AE00202
Human bladder with AE00202

Formaldehyde-fixed, paraffin-embedded human bladder stained with GLUT-1 Mouse Monoclonal Antibody AE00202 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00202 to GLUT-1 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related other proteins SLC2A2, SLC2A3, SLC2A4 and SLCA14. These proteins were part of the array used and showed no cross-reactivity signals.

AE00202 on protein array
AE00202 on HuProt(TM) array

Cross-reactivity assessment of GLUT-1 Mouse Monoclonal Antibody AE00202 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified GLUT-1 Mouse Monoclonal Antibody AE00202. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00202
AE00202 Coomassie bands

Integrity of the purified antibody AE00202 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 203-305 of human GLUT-1 protein (exact sequence is proprietary)

Epitope

Luminal domain (within aa 203-305 region)

Cellular localization

cell surface, plasma membrane

Positive Controls

bladder, breast carcinoma, MCF7

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