Description
AKT1-pS473
All names and symbols: Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; RAC-alpha serine/threonine-protein kinase; serine/threonine kinase 1; AKT; CWS6; PKB; PKB-ALPHA; PKBalpha; PRKBA; RAC; RAC-ALPHA; RAC-PK-alpha
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Confirmed species reactivity | Human, Mouse, Rat |
| Confirmed applications | IHC, WB |
| Aeonian Rating® | 70 |
| RRID | AB_2813761 |
Immunohistochemistry (IHC):
AE00207 to AKT1-pS473 was successfully used to stain nuclei in human colon, breast carcinoma and rat ovary sections. Recommended concentration: 5-15ug/ml
Formaldehyde-fixed, paraffin-embedded human colon stained with AKT1-pS473 Rabbit Polyclonal Antibody AE00207 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human breast carcinoma stained with AKT1-pS473 Rabbit Polyclonal Antibody AE00207 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded rat ovary stained with AKT1-pS473 Rabbit Polyclonal Antibody AE00207 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.
Western Blot (WB):
AE00207 to AKT1-pS473 was successfully used to stain an approx. 60kDa band in lysates of PDGF-stimulated NIH3T3. Recommended concentration: 1-3ug/ml
Western Blot of 30ug lysate from NIH3T3 with or without stimulation with 100ng/ml PDGF for 15min, stained with AKT1-pS473 Rabbit Polyclonal Antibody AE00207 at 1ug/ml (1h at ambient temp). ECL staining by HRP.
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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