BRCA1-pS1423 Polyclonal Antibody AE00209

$520,00$825,00

Phospho-specific rabbit polyclonal antibody to human BRCA1, phosphorylated S1423 (BRCA1-pS1423)

See all our BRCA1 antibodies

Official Symbol: BRCA1

GeneID: 672

Swiss-Prot: P383984

Description

BRCA1-pS1423

All names and symbols: Breast cancer type 1 susceptibility protein; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; BRCAI; BRCA-1; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman, Mouse, Rat
Confirmed applicationsICC, IHC, WB
Aeonian Rating®70
RRIDAB_2813763

Immunocytochemistry (ICC):

AE00209 to BRCA1-pS1423 was successfully used to stain nuclei in cell line U2OS. Recommended concentration: 5-15ug/ml

ICC of U2OS stained with BRCA1-pS1423 Rabbit Phospho-Specific Polyclonal Antibody AE00209
U2OS with AE00209

Confocal microscopy of U2OS stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209 at 10ug/ml (1h at ambient temp). Detection by Alexa Fluor 488 (green, top + bottom) for the antibody and DAPI (blue, bottom only) for nuclear staining.

Immunohistochemistry (IHC):

AE00209 to BRCA1-pS1423 was successfully used to stain nuclei in selective human and rodent tissue sections. Please note the staining differences side-by-side. Recommended concentration: 5-15ug/ml

IHC of human stomach and gastric cancer stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209
Human stomach and gastric cancer with AE00209

Formaldehyde-fixed, paraffin-embedded human stomach (left) and gastric cancer (right) stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

IHC of rat testis and spleen stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209
Rat testis and spleen with AE00209

Formaldehyde-fixed, paraffin-embedded rat testis (left) and rat spleen (right) stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

IHC of mouse testis and spleen stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209
Mouse testis and spleen with AE00209

Formaldehyde-fixed, paraffin-embedded mouse testis (left) and mouse spleen (right) stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

IHC of human breast cancer and liver cancer stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209
Human breast + liver cancer with AE00209

Formaldehyde-fixed, paraffin-embedded human breast cancer (left) and liver cancer (right) stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

Western Blot (WB):

AE00209 to BRCA1-pS1423 was successfully used to stain an approx. 270kDa band in lysates of anisomycin-stimulated HT29 after overnight serum-starvation.. Recommended concentration: 1-3ug/ml

WB of HT29 stained with BRCA1-pS1423 Rabbit Phospho-Specific Polyclonal Antibody AE00209
HT29 with AE00209

Western Blot of 30ug lysate from HT29 with or without 30 min stimulation by 5ug/ml Anisomycin, stained with BRCA1-pS1423 Rabbit Polyclonal Antibody AE00209 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated S1423 of human BRCA1 (NP_009225.1)

Epitope

A short sequence around phosphorylated S1423: HGSQP

Cellular localization

nucleus

Positive Controls

Anisomycin-stimulated HT29

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