ESR1-pS118 Polyclonal Antibody AE00217

$520,00$825,00

Phospho-specific rabbit polyclonal antibody to human ESR1, phosphorylated S118 (ESR1-pS118)

See all our ESR1 antibodies

Official Symbol: ESR1

GeneID 2099

Swiss-Prot: P03372

Description

ESR1-pS118

All names and symbols: Estrogen receptor 1; Estrogen receptor-1; Estrogen receptor alpha; Estrogen receptor-alpha; ERalpha; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ER; ESR; ESRA; ESTRR; Era; NR3A1

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC, WB
Aeonian Rating®70
RRIDAB_2813801

Immunohistochemistry (IHC):

AE00217 to ESR1-pS118 was successfully used to stain nuclei in epithelial cells of human breast carcinoma sections. Recommended concentration: 1-3ug/ml

IHC of human breast carcinoma stained with ESR1-pS118 Rabbit Polyclonal Antibody AE00217
Human breast carcinoma with AE00217

Formaldehyde-fixed, paraffin-embedded human breast carcinoma stained with ESR1-S118 Rabbit Polyclonal Antibody AE00217 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

Western Blot (WB):

AE00217 to ESR1-pS118 was successfully used to stain an approx. 56kDa band in lysates of MCF7 after overnight serum starvation and subsequent treatment with Calyculin A. Recommended concentration: 1-3ug/ml

WB of MCF7 stained with ESR1-pS118 Rabbit Phospho-Specific Polyclonal Antibody AE00217
MCF7 with AE00217

Western Blot of 30ug lysates from serum-starved MCF7 with or without stimulation by 100nM Calyculin A (30 min, 37C) ), stained with ESR1-S118 Rabbit Polyclonal Antibody AE00217 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated S118 of human ESR1 (NP_000116.2)

Epitope

A short sequence around phosphorylated S118 (exact sequence is proprietary)

Cellular localization

nucleus

Positive Controls

Calyculin A-treated serum-starved MCF7, ER-type breast carcinoma

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