TP53-pS33 Polyclonal Antibody AE00220

520,00825,00

Phospho-specific rabbit polyclonal antibody to human TP53, phosphorylated S33 (TP53-pS33)

See all our TP53 antibodies

Official Symbol: TP53

GeneID: 7157

Swiss-Prot: P04637

Description

TP53-pS33

All names and symbols: Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor protein p53; Tumor suppressor p53; BCC7; LFS1; P53; TRP53

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman, Mouse, Rat
Confirmed applicationsIHC, IP, WB
Aeonian Rating®70
RRIDAB_2813805

Immunohistochemistry (IHC):

AE00220 to TP53-pS33 was successfully used to show selective nuclear staining in human and rodent tissue sections. Recommended concentration: 5-15ug/ml

IHC of human tonsil and appendix stained with TP53-pS33 Rabbit Polyclonal Antibody AE00220
Human tonsil and appendix with AE00220

Formaldehyde-fixed, paraffin-embedded human tonsil (left) and appendix (right) stained with TP53-pS33 Rabbit Polyclonal Antibody AE00220 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

IHC of human colon cancer and gasric cancer stained with TP53-pS33 Rabbit Polyclonal Antibody AE00220
Colon cancer and gastric cancer with AE00220

Formaldehyde-fixed, paraffin-embedded human colon cancer (left) and gastric cancer (right) stained with TP53-pS33 Rabbit Polyclonal Antibody AE00220 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

IHC of mouse liver and rat liver stained with TP53-pS33 Rabbit Polyclonal Antibody AE00220
Mouse and rat liver with AE00220

Formaldehyde-fixed, paraffin-embedded mouse liver (left) and rat liver (right) stained with TP53-pS33 Rabbit Polyclonal Antibody AE00220 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

IHC of mouse lung and rat lung stained with TP53-pS33 Rabbit Polyclonal Antibody AE00220
Mouse and rat lung with AE00220

Formaldehyde-fixed, paraffin-embedded mouse lung (left) and rat lung (right) stained with TP53-pS33 Rabbit Polyclonal Antibody AE00220 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

Immunoprecipitation (IP):

AE00220 to TP53-pS33 was successfully used to immunoprecipitate an approx. 53kDa band from lysates of Nocodazole-treated HT29. Recommended amount: 3-6ug

IP from HT29, WB stained with TP53-pS33 Rabbit Phospho-Specific Polyclonal Antibody AE00220
IP from Ht29 with AE00220

Western Blot of immunoprecipitation (lanes 2 and 3) from 200ug HT29 (16h treated by 100ng/ml Nocodazole) extract, using 3ug TP53-pS33 Rabbit Polyclonal Antibody AE00220, and using the same antibody for staining the blot at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Western Blot (WB):

AE00220 to TP53-pS33 was successfully used to stain an approx. 55kDa band in lysates of HT29 after 16h Nocodazole treatment. Recommended concentration: 1-3ug/ml

WB of HT29 stained with TP53-pS33 Rabbit Phospho-Specific Polyclonal Antibody AE00220
HT29 with AE00220

Western Blot of HT29 (30ug) with or without Nocodazole (100ng/ml, 16h) treatment, stained with TP53-pS33 Rabbit Polyclonal Antibody AE00220 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated S33 of human TP53 (NP_000537.3)

Epitope

A short sequence around phosphorylated S33: VLSPL

Cellular localization

nucleus

Positive Controls

Nocodazole-treated HT29

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