ERBB4-pY1284 Polyclonal Antibody AE00227

$520,00$825,00

Phospho-specific rabbit polyclonal antibody to HER4, phosphorylated Y1284 (ERBB4-pY1284)

Official Symbol: ERBB4

GeneID: 2066

Swiss-Prot: Q15303

Description

ERBB4-pY1284

All names and symbols: Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; erb-b2 receptor tyrosine kinase 4; ERBB4; HER4; ALS19; p180erbB4

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsWB
Aeonian Rating®70
RRIDAB_2814723

Western Blot (WB):

AE00227 to ERBB4-pY1284 was successfully used to stain an approx. 200kDa band in lysates of serum-starved A431 after an EGF treatment. Recommended concentration: 1-3ug/ml

WB of A431 stained with ERBB4-pY1284 Rabbit Phospho-Specific Polyclonal Antibody AE00227
A431 with AE00227

Western Blots of 30ug lysates of A431 with and without EGF (100ng/ml, 30min) treatment after overnight serum-starvation, stained with ERBB4-pY1284 Rabbit Polyclonal Antibody AE00227 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated Y1284 of human HER4 (NP_005226.1).

Epitope

A short sequence around phosphorylated Y1284: PEYLS

Cellular localization

cell surface, plasma membrane

Positive Controls

EGF-stimulated serum-starved A431

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