VIM-pS83 Polyclonal Antibody AE00238

$520,00$825,00

Phospho-specific rabbit polyclonal antibody to Vimentin, phosphorylated S83 (VIM-pS83)

See all our Vimentin antibodies

Official Symbol: VIM

GeneID: 7431

Swiss-Prot: P08670

Description

VIM-pS83

All names and symbols: Vimentin; VIM

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsWB
Aeonian Rating®70
RRIDAB_2814733

Western Blot (WB):

AE00238 to VIM-pS83 was successfully used to stain an approx. 62kDa band in lysates of HeLa after Paclitaxel-treatment. Recommended concentration: 1-3ug/ml

WB of HeLa stained with VIM-pS83 Rabbit Phospho-Specific Polyclonal Antibody AE00238
HeLa with AE00238

Western Blots of 30ug lysates of HeLa (4mM Hydroxyurea 20h or 100nM Paclitaxel 20h), stained with VIM-pS83 Rabbit Polyclonal Antibody AE00238 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated S83 of human Vimentin (NP_003371.2)

Epitope

A short sequence around phosphorylated S83: QDSVD

Cellular localization

cytoskeleton, intermediate filaments, perinuclear

Positive Controls

Paclitaxel-treated HeLa

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