ZAP70 Monoclonal Antibody AE00240

$520,00

Selective and specific mouse monoclonal antibody clone ZAP70/2035 to human ZAP70

See all our ZAP70 antibodies

Official Symbol: ZAP70

GeneID: 7535

Swiss-Prot: P43403

Description

ZAP70

All names and symbols: 70kDa zeta-chain associated protein; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Zeta chain of T cell receptor Associated Protein kinase 70; ADMIO2; IMD48;; SRK; STCD; STD; TZK; ZAP-70

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa



Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA
Aeonian Rating®82
RRIDAB_2814735



Immunocytochemistry (ICC):

AE00240 to ZAP70 was successfully used to stain cytoplasm in cell line Jurkat. Recommended concentration: 1-3ug/ml

ICC of Jurkat stained with ZAP70 mouse Monoclonal Antibody AE00240
Jurkat with AE00240

Confocal microscopy of cell line jurkat stained with ZAP70 Mouse Monoclonal Antibody AE00240 at 1-2ug/ml (1h at ambient temp). CF488 (green) staining of the antibody and RedDot (red) for nuclear staining.

Immunohistochemistry (IHC):

AE00240 to ZAP70 was successfully used to stain T-cells mainly outside the Germinal Centres in human tonsil sections. Recommended concentration: 1-3ug/ml

IHC of human tonsil stained with ZAP70 Mouse Monoclonal Antibody AE00240
Human tonsil with AE00240

Formaldehyde-fixed, paraffin-embedded human prostate carcinoma stained with ZAP70 Mouse Monoclonal Antibody AE00240 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00240 to ZAP70 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed closely related protein SYK. This protein was part of the array used and showed no cross-reactivity signals.

AE00240 on protein array
AE00240 on HuProt(TM) array

Cross-reactivity assessment of ZAP70 Mouse Monoclonal Antibody AE00240 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified ZAP70 Mouse Monoclonal Antibody AE00240. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00240
AE00240 Coomassie bands

Integrity of the purified antibody AE00240 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 24kDa resp. (R).

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant fragment around aa 247-382 of human ZAP70 (exact sequence is proprietary)

Epitope

Within aa 247-382 region

Cellular localization

cytoplasm, transported to the plasma membrane upon TCR activation

Positive Controls

Jurkat, lymph node, tonsil

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