CD11b Monoclonal Antibody AE00279

520,00

Selective and specific mouse monoclonal antibody clone ITGAM/3340 to human CD11b

See all our CD11b antibodies

Official Symbol: ITGAM

GeneID: 3684

Swiss-Prot: P11215

Description

CD11b

All names and symbols: CD11 antigen-like family member B; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CR3A; MO1A; CD11B; ITGAM; MAC-1; MAC1A; SLEB6

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa



Confirmed species reactivityHuman
Confirmed applicationsIHC, PA, WB
Aeonian Rating®83
RRIDAB_2819071



Immunohistochemistry (IHC):

AE00279 to CD11b was successfully used to stain membranes in human tonsil sections. Recommended concentration: 1-3ug/ml

IHC of human tonsil stained with CD11b Mouse Monoclonal Antibody AE00279
Human tonsil with AE00279

Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD11b Mouse Monoclonal Antibody AE00279 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00279 to CD11b was successfully used to stain bands between 110 and 140kDa in human spleen lysates. Recommended concentration: 0.3-1ug/ml

WB of human spleen stained with CD11b Mouse Monoclonal Antibody AE00279
Human spleen with AE00279

Western Blot of human spleen lysate (30ug) stained with CD11b Mouse Monoclonal Antibody AE00279 at 0.5ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00279 to CD11b were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related proteins: ITGAX (CD11c) and ITGAD (CD11d). These proteins were part of the array used and showed no cross-reactivity signals.

AE00279 on protein array
AE00279 on HuProt(TM) array

Cross-reactivity assessment of CD11b Mouse Monoclonal Antibody AE00279 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified CD11b Mouse Monoclonal Antibody AE00279. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00279
AE00279 Coomassie bands

Integrity of the purified antibody AE00279 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R).

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Aeonian Rating®

83

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 941-1074 of human CD11b protein (exact sequence is proprietary)

Epitope

Extracellular domain (within aa 941-1074 region)

Cellular localization

cell surface, plasma membrane

Positive Controls

granulocytes, lymph node, macrophages, monocytes, spleen, tonsil

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