ZAP70-pY493 Polyclonal Antibody AE00241

$520,00$825,00

Phospho-specific rabbit polyclonal antibody to ZAP70, phosphorylated Y493 (ZAP70-pY493)

See all our ZAP70 antibodies

Official Symbol: ZAP70

GeneID: 7535

Swiss-Prot: P43403

Description

ZAP70-pY493

All names and symbols: 70kDa zeta-chain associated protein; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Zeta chain of T cell receptor Associated Protein kinase 70; ADMIO2; IMD48;; SRK; STCD; STD; TZK; ZAP-70

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsWB
Aeonian Rating®70
RRIDAB_2814736

Western Blot (WB):

AE00241 to ZAP70-pY493 was successfully used to stain an approx. 62kDa band in lysates of Jurkat after PMA/TPA, Anisomycin and ATP-treatment. Recommended concentration: 1-3ug/ml

WB of Jurkat stained with ZAP70-pY493 Rabbit Phospho-Specific Polyclonal Antibody AE00241
Jurkat with AE00241

Western Blots of 30ug lysates of Jurkat (200nM PMA/TPA 10min, or 5ug/ml Anisomycin 30min, or 5mM ATP 1h), stained with ZAP70-pY493 Rabbit Polyclonal Antibody AE00241 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated Y493 of human ZAP70 (NP_001070.2)

Epitope

A short sequence around phosphorylated Y493 (exact sequence is proprietary)

Cellular localization

cytoplasm, transported to the plasma membrane upon TCR activation

Positive Controls

Anisomycin-treated Jurkat, ATP-treated Jurkat

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