AKT1-pS129 Polyclonal Antibody AE00297

520,00825,00

Phospho-specific rabbit polyclonal antibody to AKT1, phosphorylated S129 (AKT1-pS129)

See all our AKT1 antibodies

Official Symbol: AKT1

GeneID: 207

Swiss-Prot: P31749

Description

AKT1-pS129

All names and symbols: Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; RAC-alpha serine/threonine-protein kinase; serine/threonine kinase 1; AKT; CWS6; PKB; PKB-ALPHA; PKBalpha; PRKBA; RAC; RAC-ALPHA; RAC-PK-alpha

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsWB
Aeonian Rating®70
RRIDAB_2892787

Western Blot (WB):

AE00297 to AKT1-S129 was successfully used to stain an approx. 65kDa band in PMA-treated A549 lysates. Recommended concentration: 1-3ug/ml

WB of PMA-treated cell line A549 stained with AKT1-pS124 Rabbit Polyclonal Antibody AE00297
PMA-treated A549 with AE00297

Western Blot of 10ug PMA-treated A549 lysates stained with AKT1-S129 Rabbit Polyclonal Antibody AE00297 at 1ug/ml (overnight at 4℃) in the presence (+) or absence (-) of its antigen (P peptide) or its non-phosphorylated control (C peptide). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated S129 of human AKT1 (NP_005154.2)

Epitope

A short sequence around phosphorylated S129 (exact sequence is proprietary)

Cellular localization

intracellular vesicles, nucleus, plasma membrane

Positive Controls

PMA-treated A549

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