B7-H6 Recombinant Antibody AE00326

$520,00

Functional and specific rabbit recombinant antibody clone 17B1.3R to human B7-H6

See all our B7-H6 antibodies

Official Symbol: NCR3LG1

GeneID: 35374383

Swiss-Prot: Q68D85

Description

B7-H6

All names and symbols: Natural cytotoxicity triggering receptor 3 ligand 1; natural killer cell cytotoxicity receptor 3 ligand 1; B7 homolog 6; B7H6; B7-H6; DKFZp686O24166

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG, kappa, recombinant chimeric version of mouse IgG1 clone 17B1.3
Confirmed species reactivityHuman
Confirmed applicationsFC, ICC, blocking
Aeonian Rating®90
RRIDAB_2909610

Immunoassays

Immunocytochemistry (ICC):

AE00326 to B7-H6 was successfully used to stain the cellular membrane in K562. Recommended concentration: 5-15ug/ml.

ICC of K562 stained with B7-H6 Rabbit Recombinant Antibody AE00326
K562 with AE00326

Confocal micrroscopy of PFA-fixed K562 cells stained with B7-H6 Rabbit Recombinant Antibody AE00326 at 10ug/ml for 1h at RT. Detection by CF488 (green) for the antibody and DAPI (blue) for nuclear staining. Bottom right shows staining with an isotype control antibody.

Flow Cytometry (FC):

The original clone 17B1.3 was successfully used on PBMCs, gated for CD45+CD14+CD19-CD3- monocytes from sepsis patients, and on CD45+CD14+ monocytes stimulated by IL-1β.

Matta J, Baratin M, Chiche L, Forel JM, Cognet C, Thomas G, Farnarier C, Piperoglou C, Papazian L, Chaussabel D, Ugolini S, Vély F, Vivier E. Induction of B7-H6, a ligand for the natural killer cell-activating receptor NKp30, in inflammatory conditions. Blood. 2013 Jul 18;122(3):394-404. doi: 10.1182/blood-2013-01-481705. PMID: 23687088.

Biological Activity

Blocking activation of NKp30+ DOMsp30 reporter cells:

The original clone 17B1.3 was successfully used to block the CD45+CD14+ monocytic activation of NKp30 cells.

Kim et al. Immunoregulatory effects of a monoclonal antibody to human 4-1 bb molecule on alloantigen-mediated immune rMatta J, Baratin M, Chiche L, Forel JM, Cognet C, Thomas G, Farnarier C, Piperoglou C, Papazian L, Chaussabel D, Ugolini S, Vély F, Vivier E. Induction of B7-H6, a ligand for the natural killer cell-activating receptor NKp30, in inflammatory conditions. Blood. 2013 Jul 18;122(3):394-404. doi: 10.1182/blood-2013-01-481705. PMID: 23687088.

17B1.3-specific most recent literature

Xu X, Narni-Mancinelli E, Cantoni C, Li Y, Guia S, Gauthier L, Chen Q, Moretta A, Vély F, Eisenstein E, Rangarajan S, Vivier E, Mariuzza RA. Structural Insights into the Inhibitory Mechanism of an Antibody against B7-H6, a Stress-Induced Cellular Ligand for the Natural Killer Cell Receptor NKp30. J Mol Biol. 2016 Nov 6;428(22):4457-4466. doi: 10.1016/j.jmb.2016.09.011. PMID: 27663271.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant soluble B7-H6 (aa25-262)

Epitope

aa 74–115, extracellular, away from the NKp30 receptor binding site (Xu et al)

Cellular localization

plasma membrane

Positive Controls

carcinoma, HeLa, IL-1b activated CD45+CD14+ monocytes, K562

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.