Description
B7-H6
All names and symbols: Natural cytotoxicity triggering receptor 3 ligand 1; natural killer cell cytotoxicity receptor 3 ligand 1; B7 homolog 6; B7H6; B7-H6; DKFZp686O24166
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG, kappa, recombinant chimeric version of mouse IgG1 clone 17B1.3 |
| Confirmed species reactivity | Human |
| Confirmed applications | FC, ICC, blocking |
| Aeonian Rating® | 90 |
| RRID | AB_2909610 |
Immunoassays
Immunocytochemistry (ICC):
AE00326 to B7-H6 was successfully used to stain the cellular membrane in K562. Recommended concentration: 5-15ug/ml.
Confocal micrroscopy of PFA-fixed K562 cells stained with B7-H6 Rabbit Recombinant Antibody AE00326 at 10ug/ml for 1h at RT. Detection by CF488 (green) for the antibody and DAPI (blue) for nuclear staining. Bottom right shows staining with an isotype control antibody.
Flow Cytometry (FC):
The original clone 17B1.3 was successfully used on PBMCs, gated for CD45+CD14+CD19-CD3- monocytes from sepsis patients, and on CD45+CD14+ monocytes stimulated by IL-1β.
Matta J, Baratin M, Chiche L, Forel JM, Cognet C, Thomas G, Farnarier C, Piperoglou C, Papazian L, Chaussabel D, Ugolini S, Vély F, Vivier E. Induction of B7-H6, a ligand for the natural killer cell-activating receptor NKp30, in inflammatory conditions. Blood. 2013 Jul 18;122(3):394-404. doi: 10.1182/blood-2013-01-481705. PMID: 23687088.
Biological Activity
Blocking activation of NKp30+ DOMsp30 reporter cells:
The original clone 17B1.3 was successfully used to block the CD45+CD14+ monocytic activation of NKp30 cells.
Kim et al. Immunoregulatory effects of a monoclonal antibody to human 4-1 bb molecule on alloantigen-mediated immune rMatta J, Baratin M, Chiche L, Forel JM, Cognet C, Thomas G, Farnarier C, Piperoglou C, Papazian L, Chaussabel D, Ugolini S, Vély F, Vivier E. Induction of B7-H6, a ligand for the natural killer cell-activating receptor NKp30, in inflammatory conditions. Blood. 2013 Jul 18;122(3):394-404. doi: 10.1182/blood-2013-01-481705. PMID: 23687088.
17B1.3-specific most recent literature
Xu X, Narni-Mancinelli E, Cantoni C, Li Y, Guia S, Gauthier L, Chen Q, Moretta A, Vély F, Eisenstein E, Rangarajan S, Vivier E, Mariuzza RA. Structural Insights into the Inhibitory Mechanism of an Antibody against B7-H6, a Stress-Induced Cellular Ligand for the Natural Killer Cell Receptor NKp30. J Mol Biol. 2016 Nov 6;428(22):4457-4466. doi: 10.1016/j.jmb.2016.09.011. PMID: 27663271.
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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