BAX Recombinant Antibody AE00329

Description

BAX (active monomer)

All names and symbols: Apoptosis regulator BAX; BCL2 associated X, apoptosis regulator; Bcl-2-like protein 4; Bcl2-L-4; BCL2L4

Host species	Rabbit
Clonality	Recombinant
Isotype	IgG, kappa, recombinant chimeric version of mouse IgG1 clone 6A7

Confirmed species reactivity	Human, Mouse, Rat
Confirmed applications	FC, ICC, IP
Aeonian Rating®	100
RRID	AB_2909613

Immunoassays

Flow Cytometry (FC):

The original clone 6A7 was successfully used in etoposide-treated mouse embryonic fibroblast cells.

The original clone 6A7 was successfully used in etoposide-treated mouse embryonic fibroblast cells.
Dengler MA, Gibson L, Adams JM. BAX mitochondrial integration is regulated allosterically by its α1-α2 loop. Cell Death Differ. 2021 Jun 16. doi: 10.1038/s41418-021-00815-x. PMID: 34135480.

ImmunoCytoChemistry (ICC):

The original clone 6A7 was successfully used to detect monomeric active BAX in MN9D cells.

Chung Y, Kim Y, Yun N, Oh YJ. Dysregulated autophagy is linked to BAX oligomerization and subsequent cytochrome c release in 6-hydroxydopmaine-treated neuronal cells. Biochem Biophys Res Commun. 2021 Apr 9;548:20-26. doi: 10.1016/j.bbrc.2021.02.045. PMID: 33631669.

ImmunoPrecipitation (IP):

The original clone 6A7 was successfully used when coupled to cepharose beads for immunoprecipitation from murine thymocyte lysates in the presence of Triton X100 or NP40. Without TX100 or NP40, IP does not work.

Hsu YT, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997 May 23;272(21):13829-34. doi: 10.1074/jbc.272.21.13829. PMID: 9153240.

Biological Activity

Monomeric Specificity:

The original clone 6A7 binds in the presence of non-ionic detergent exclusively to the monomer of BAX, and not to Bax complexed with either Bcl-XL or Bcl-2.

Hsu YT, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997 May 23;272(21):13829-34. doi: 10.1074/jbc.272.21.13829. PMID: 9153240.

6A7-specific most recent literature

Al Rahim M, Thatipamula S, Pasinetti GM, Hossain MA. Neuronal Pentraxin 1 Promotes Hypoxic-Ischemic Neuronal Injury by Impairing Mitochondrial Biogenesis via Interactions With Active Bax[6A7] and Mitochondrial Hexokinase II. ASN Neuro. 2021 Jan-Dec;13:17590914211012888. doi: 10.1177/17590914211012888. PMID: 34098747.

Blombery P, Lew TE, Dengler MA, Thompson ER, Lin VS, Chen X, Nguyen T, Panigrahi A, Handunnetti SM, Carney D, Westerman DA, Tam CS, Adams JM, Wei AH, Huang DCS, Seymour JF, Roberts A, Anderson MA. Clonal hematopoiesis, myeloid disorders and BAX-mutated myelopoiesis in patients receiving venetoclax for CLL. Blood. 2021 Sep 1:blood.2021012775. doi: 10.1182/blood.2021012775. PMID: 34469514.

Seiller C, Maiga S, Touzeau C, Bellanger C, Kervoëlen C, Descamps G, Maillet L, Moreau P, Pellat-Deceunynck C, Gomez-Bougie P, Amiot M. Dual targeting of BCL2 and MCL1 rescues myeloma cells resistant to BCL2 and MCL1 inhibitors associated with the formation of BAX/BAK hetero-complexes. Cell Death Dis. 2020 May 5;11(5):316. doi: 10.1038/s41419-020-2505-1. PMID: 32371863.

Wang TS, Coppens I, Saorin A, Brady NR, Hamacher-Brady A. Endolysosomal Targeting of Mitochondria Is Integral to BAX-Mediated Mitochondrial Permeabilization during Apoptosis Signaling. Dev Cell. 2020 Jun 22;53(6):627-645.e7. doi: 10.1016/j.devcel.2020.05.014. PMID: 32504557.

Lindenboim L, Grozki D, Amsalem-Zafran AR, Peña-Blanco A, Gundersen GG, Borner C, Hodzic D, Garcia-Sáez AJ, Worman HJ, Stein R. Apoptotic stress induces Bax-dependent, caspase-independent redistribution of LINC complex nesprins. Cell Death Discov. 2020 Sep 18;6(1):90. doi: 10.1038/s41420-020-00327-6. PMID: 33024575.

Expiration	Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructions	Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
Warranty	This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
Liability	This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.