CD276 Recombinant Antibody AE00324

520,00

Functional and selective rabbit recombinant antibody clone MJ18R to mouse CD276 (B7-H3)

See all our CD276 antibodies

Official Symbol: Cd276

GeneID: 102657

Swiss-Prot: Q8VE98

Description

CD276 (mouse)

All names and symbols: B7 homolog 3; B7H3; B7-H3; B7RP; B7RP-2; AU016588; 6030411F23Rik

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG, kappa, recombinant chimeric version of rat IgG1 clone MJ18
Confirmed species reactivityMouse
Confirmed applicationsELISA, FC, enhancement CD8+ T-cell tumour infiltration, inhibition CD4+ T-cell proliferation
Aeonian Rating®90
RRIDAB_2904612

Immunoassays

Enzyme-Linked ImmunoSorbent Assay (ELISA):

AE00324 to CD276 was successfully used antigen-coated microwells.

ELISA of B7-H3-Fc fusion protein stained with CD276 Rabbit Recombinant Antibody AE00324
B7-H3-Fc fusion protein with AE00324

Binding curve of serially diluted B7-H3 Rabbit Recombinant Antibody AE00324 to microwells coated with 5 µg/ml mouse B7-H3-Fc fusion protein. HRP-mediated detection from secondary anti-rabbit antibody.

Flow Cytometry (FC):

The selectivity of the original clone MJ18 was confirmed by comparing transfectant L5178Y and P815 cell lines, each expressing a different member of the B7 protein family.

Nagashima O, Harada N, Usui Y, Yamazaki T, Yagita H, Okumura K, Takahashi K, Akiba H. B7-H3 contributes to the development of pathogenic Th2 cells in a murine model of asthma. J Immunol. 2008 Sep 15;181(6):4062-71. doi: 10.4049/jimmunol.181.6.4062. PMID: 18768862.

Biological Activity

Inhibition CD4+ T cell proliferation:

The original clone MJ18 was successfully used to inhibit the OVA323–339-induced proliferation of CD4+ T cells in the presence of bone marrow dendritic cells.

Nagashima O, Harada N, Usui Y, Yamazaki T, Yagita H, Okumura K, Takahashi K, Akiba H. B7-H3 contributes to the development of pathogenic Th2 cells in a murine model of asthma. J Immunol. 2008 Sep 15;181(6):4062-71. doi: 10.4049/jimmunol.181.6.4062. PMID: 18768862.

Enhancement of CD8+ T cell infiltration into tumours:

The original clone MJ18 was successfully used to block B7-H3, thus enhancing CD8+ T cells infiltration into pancraatic tumours in mice.

Yamato I, Sho M, Nomi T, Akahori T, Shimada K, Hotta K, Kanehiro H, Konishi N, Yagita H, Nakajima Y. Clinical importance of B7-H3 expression in human pancreatic cancer. Br J Cancer. 2009 Nov 17;101(10):1709-16. doi: 10.1038/sj.bjc.6605375. PMID: 19844235.

MJ18-specific most recent literature

Masemann D, Meissner R, Schied T, Lichty BD, Rapp UR, Wixler V, Ludwig S. Synergistic anti-tumor efficacy of oncolytic influenza viruses and B7-H3 immune- checkpoint inhibitors against IC-resistant lung cancers. Oncoimmunology. 2021 Feb 17;10(1):1885778. doi: 10.1080/2162402X.2021.1885778. PMID: 33643696.

Cheng N, Bei Y, Song Y, Zhang W, Xu L, Zhang W, Yang N, Bai X, Shu Y, Shen P. B7-H3 augments the pro-angiogenic function of tumor-associated macrophages and acts as a novel adjuvant target for triple-negative breast cancer therapy. Biochem Pharmacol. 2021 Jan;183:114298. doi: 10.1016/j.bcp.2020.114298. Epub 2020 Oct 22. PMID: 33153969.

Mao L, Fan TF, Wu L, Yu GT, Deng WW, Chen L, Bu LL, Ma SR, Liu B, Bian Y, Kulkarni AB, Zhang WF, Sun ZJ. Selective blockade of B7-H3 enhances antitumour immune activity by reducing immature myeloid cells in head and neck squamous cell carcinoma. J Cell Mol Med. 2017 Sep;21(9):2199-2210. doi: 10.1111/jcmm.13143. PMID: 28401653.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

The extracellular domain (aa 1–242) of mouse B7-H3 linked to the Fc portion of mouse IgG2a.

Epitope

The extracellular domain (aa 1–242)

Cellular localization

cell surface, plasma membrane

Positive Controls

carcinoma

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