Podoplanin (MAP Epitope Tag) Recombinant Antibody AE00338

Description

Podoplanin (Map epitope tag)

RANDAM-2; T1-alpha; T1alpha; E11; Gp38; OTS-8; Pdpn; T1a

The Map tag is used as epitope tag for the detection of recombinant proteins tagged with the Map tag sequence.

Host species	Rat
Clonality	Recombinant
Isotype	IgG2a, kappa, recombinant version of clone PMab-1

Confirmed species reactivity	Mouse
Confirmed applications	IAP, IHC, IP, WB
Aeonian Rating®	100
RRID	AB_2910189

ImmunoAffinity Purification (IAP):

The original clone PMab-1 was successfully used to purify MAP-tagged ATRX (aa 2273–2413) from E.coli lysates and soluble forms of EGFR and HER2 from LN229 supernatants.

Fujii Y, Kaneko MK, Kato Y. MAP Tag: A Novel Tagging System for Protein Purification and Detection. Monoclon Antib Immunodiagn Immunother. 2016 Dec;35(6):293-299. doi: 10.1089/mab.2016.0039. PMID: 27801621.

ImmunoHistoChemistry (IHC):

The original clone PMab-1 was successfully used to stain sections of mouse kidney, lung, and sublingual gland tissues, consistently with another Podoplanin monoclonal antibody.

Kaji C, Tsujimoto Y, Kato Kaneko M, Kato Y, Sawa Y. Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin. Acta Histochem Cytochem. 2012 Aug 30;45(4):227-37. doi: 10.1267/ahc.12008. PMID: 23012488.

ImmunoPrecipitation (IP):

The original clone PMab-1 was successfully used to pull down internally MAP-tagged Fibronectin, using PMab-1-Sepharose beads.

Wakasa A, Kaneko MK, Kato Y, Takagi J, Arimori T. Site-specific epitope insertion into recombinant proteins using the MAP tag system. J Biochem. 2020 Oct 1;168(4):375-384. doi: 10.1093/jb/mvaa054. PMID: 32386302.

Western Blot (WB):

The original clone PMab-1 was successfully used to detect endogenous Podoplanin in B16-F10 lysates.

Kaji C, Tsujimoto Y, Kato Kaneko M, Kato Y, Sawa Y. Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin. Acta Histochem Cytochem. 2012 Aug 30;45(4):227-37. doi: 10.1267/ahc.12008. Epub 2012 Jul 25. PMID: 23012488

Expiration	Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructions	Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
Warranty	This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
Liability	This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.