Description
SV5-Pk (V5) epitope tag
Epitope tag, also known as GKPIPNPLLGLDST tag; V5 epitope tag; GKPIPNPLLGLDST epitope tag; Pk tag; Pk-tag, for the detection of recombinant proteins tagged with the GKPIPNPLLGLDST sequence.
| Host species | Rat |
| Clonality | Recombinant |
| Isotype | IgG2a, kappa, recombinant chimeric version of mouse IgG1 clone SV5-Pk |
| Confirmed applications | WB |
| Aeonian Rating® | 100 |
| RRID | AB_2910192 |
Western Blot (WB):
The original clone SV5-Pk was successfully used to detect Pk-tagged Fibronectin in transgenic mouse kidney lysates, and V5-tagged ANO5 in COS1 lysates.
Bakeberg JL, Tammachote R, Woollard JR, Hogan MC, Tuan HF, Li M, van Deursen JM, Wu Y, Huang BQ, Torres VE, Harris PC, Ward CJ. Epitope-tagged Pkhd1 tracks the processing, secretion, and localization of fibrocystin. J Am Soc Nephrol. 2011 Dec;22(12):2266-77. doi: 10.1681/ASN.2010111173. PMID: 22021705.
Vihola A, Luque H, Savarese M, Penttilä S, Lindfors M, Leturcq F, Eymard B, Tasca G, Brais B, Conte T, Charton K, Richard I, Udd B. Diagnostic anoctamin-5 protein defect in patients with ANO5-mutated muscular dystrophy. Neuropathol Appl Neurobiol. 2018 Aug;44(5):441-448. doi: 10.1111/nan.12410. PMID: 28489263.
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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