ACE2 Recombinant Antibody AE00343

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE919R to human ACE2

Official Symbol: ACE2

GeneID: 59272

Swiss-Prot: Q9BYF1

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

ACE2

All names and symbols: Angiotensin converting enzyme 2; Angiotensin-converting enzyme homolog; Angiotensin-converting enzyme-related carboxypeptidase; ACE-related carboxypeptidase; Coronavirus receptor; Metalloprotease MPROT15; ACE2; ACEH

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®95
RRIDAB_2916347

Immunohistochemistry (IHC):

AE00343 to ACE2 was successfully used to stain epithelial surface of human duodenum sections, proximal tubuli in human kidney sections, and Leydig cells plus spermatocytes in human testis sections. Recommended concentration: 1-3ug/ml

IHC of human duodenum carcinoma stained with ACE2 Rabbit Recombinant Antibody AE00343
Human duodenum with AE00343

Formaldehyde-fixed, paraffin-embedded human duodenum stained with ACE2 Rabbit Recombinant Antibody AE00343 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human kidney stained with ACE2 Rabbit Recombinant Antibody AE00343
Human kidney with AE00343

Formaldehyde-fixed, paraffin-embedded human kidney stained with ACE2 Rabbit Recombinant Antibody AE00343 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human testis stained with ACE2 Rabbit Recombinant Antibody AE00343
Human testis with AE00343

Formaldehyde-fixed, paraffin-embedded human testis stained with ACE2 Rabbit Recombinant Antibody AE00343 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
cardial endotheliumadrenal glandadipose
corpus luteumappendix mucosaaorta, media
duodenum mucosacolonbone marrow
endothelial cellsepididymisbreast
gallbladderfallopian tubecerebellum
ileum mucosaheart musclecerebrum
renal proximal tubulimyometriumduodenum Brunner gland
testispancreasendocervix
placentaendometrium
rectum mucosaepidermis
renal medulaesophagus squamous epithelium
seminal vesiclesliver
stomachlung
thyroid glandlymph node
ovarian stroma
parathyroid
parotid gland
pituitary gland
prostate
renal medulla
skeletal muscle
skin
spleen
thymus
tonsil
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
adrenocortical adenomacholangiocarcinomabreast cancer no special type
capillaries in ACE2-negative neuroendocrine tumourprostate adenocarcinomaschromophobe renal cell carcinoma
capillaries in ACE2-negative thyroid medullary carcinomaurothelial carcinomahigh-grade serous carcinoma
clear cell renal cell carcinomarenal oncocytoma
colorectal adenocarcinomaseminoma
esophageal adenocarcinoma
gastric adenocarcinoma
hepatic cholangiocellular carcinoma
papillary renal cell carcinomas
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 705-805 of human ACE2 (exact sequence is proprietary)

Epitope

aa 705-805

Cellular localization

cell surface, secreted

Positive Controls

duodenum, endothelial cells, gallbladder, kidney, ovary, renal carcinoma, small intestine, testis

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