Description
Calponin 1
All names and symbols: Basic calponin; Calponin 1; Calponin-1; Calponin H1, smooth muscle; CNN1; HEL-S-14; SMCC; Sm-Calp
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 95 |
| RRID | AB_2916351 |
Immunohistochemistry (IHC):
AE00347 to Calponin was successfully used to stain cytoskeleton in smooth muscle cells of aorta media, epididymis, and myometrium sections. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human aorta media stained with Calponin 1 Rabbit Recombinant Antibody AE00347 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human epididymis stained with Calponin 1 Rabbit Recombinant Antibody AE00347 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human myometrium stained with Calponin 1 Rabbit Recombinant Antibody AE00347 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| adrenal gland large vein | breast myoepithelial cells | adipose |
| aorta, media | colon mucosa | appendix mucosa |
| appendix muscular wall | duodenum mucosa | bone marrow |
| bronchus mucosa | kidney medulla, arterioles | cerebellum |
| colon muscular wall | lung | cerebrum |
| epididymis | pancreas arterioles | duodenum Brunner gland |
| fallopian tube | parotid gland | ectocervix |
| gallbladder | spleen trabecular smooth muscle | endocervix |
| myometrium | stomach | endometrium |
| seminal vesicle | testis with abnormal spermatogenesis | esophageal squamous epithelium |
| urinary bladder muscular wall | thyroid gland | heart muscle |
| ileum | ||
| liver | ||
| lymph node | ||
| ovarian stroma | ||
| parathyroid | ||
| pituitary gland | ||
| pregnant uterus | ||
| placenta | ||
| rectum mucosa | ||
| renal cortex | ||
| skin (weak non-spec) | ||
| testis | ||
| thymus | ||
| tonsil | ||
| urothelium |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| prostate adenocarcinoma | epithelial tumor cells | |
| gastric cancer | ||
| urothelial carcinoma | ||
| tumors derived from smooth muscle cells |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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