Calponin 1 Recombinant Antibody AE00347

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE455R to human Calponin 1

Official Symbol: CNN1

GeneID: 1264

Swiss-Prot: P51911

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

Calponin 1

All names and symbols: Basic calponin; Calponin 1; Calponin-1; Calponin H1, smooth muscle; CNN1; HEL-S-14; SMCC; Sm-Calp

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®95
RRIDAB_2916351

Immunohistochemistry (IHC):

AE00347 to Calponin was successfully used to stain cytoskeleton in smooth muscle cells of aorta media, epididymis, and myometrium sections. Recommended concentration: 1-3ug/ml

IHC of human aorta media stained with Calponin 1 Rabbit Recombinant Antibody AE00347
Human aorta media with AE00347

Formaldehyde-fixed, paraffin-embedded human aorta media stained with Calponin 1 Rabbit Recombinant Antibody AE00347 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human epididymis stained with Calponin 1 Rabbit Recombinant Antibody AE00347
Human epididymis with AE00347

Formaldehyde-fixed, paraffin-embedded human epididymis stained with Calponin 1 Rabbit Recombinant Antibody AE00347 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human myometrium stained with Calponin 1 Rabbit Recombinant Antibody AE00347
Human myometrium with AE00347

Formaldehyde-fixed, paraffin-embedded human myometrium stained with Calponin 1 Rabbit Recombinant Antibody AE00347 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
adrenal gland large veinbreast myoepithelial cellsadipose
aorta, mediacolon mucosaappendix mucosa
appendix muscular wallduodenum mucosabone marrow
bronchus mucosakidney medulla, arteriolescerebellum
colon muscular walllungcerebrum
epididymispancreas arteriolesduodenum Brunner gland
fallopian tubeparotid glandectocervix
gallbladderspleen trabecular smooth muscleendocervix
myometriumstomachendometrium
seminal vesicletestis with abnormal spermatogenesisesophageal squamous epithelium
urinary bladder muscular wallthyroid glandheart muscle
ileum
liver
lymph node
ovarian stroma
parathyroid
pituitary gland
pregnant uterus
placenta
rectum mucosa
renal cortex
skin (weak non-spec)
testis
thymus
tonsil
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
prostate adenocarcinomaepithelial tumor cells
gastric cancer
urothelial carcinoma
tumors derived from smooth muscle cells
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Synthetic peptide corresponding to the C-terminus of human Calponin

Epitope

C-terminus

Cellular localization

cytoskeleton

Positive Controls

smooth muscle cells

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