Description
CD3e
All names and symbols: CD_antigen: CD3e; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; IMD18; T3E; TCRE
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 100 |
| RRID | AB_2916353 |
Immunohistochemistry (IHC):
AE00349 to CD3e was successfully used to stain plasma membranes of T-cells in human duodenum, spleen and thymus sections. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human duodenum stained with CD3e Rabbit Recombinant Antibody AE00349 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human spleen stained with CD3e Rabbit Recombinant Antibody AE00349 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human thymus stained with CD3e Rabbit Recombinant Antibody AE00349 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| appendix mucosa | appendix muscular wall | adipose |
| duodenum mucosa | bone marrow | adrenal gland |
| gallbladder epithelium | bronchus mucosa | aorta, media |
| ileum | colon mucosa | breast |
| lymph node | duodenum Brunner gland | cerebellum |
| rectal mucosa | ectocervix | cerebrum |
| sinus paranasalis | endocervix | colon muscular wall |
| spleen | endometrium | epididymis |
| thymus | esophagus squamous epithelium | heart muscle |
| tonsil | fallopian tube | myometrium |
| renal medulla | ovarian stroma | |
| liver | parathyroid gland | |
| lung | pituitary gland | |
| pancreas | placenta | |
| paratid gland | renal cortex | |
| prostate | skeletal muscle | |
| seminal vesicle | testis | |
| skin | thyroid gland | |
| stomach | urinary bladder muscular wall | |
| urothelium |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| Anaplastic Large Cell Lymphoma | breast invasive cancer, no special type | renal oncocytoma |
| AngioImmunoblastic T-cell Lymphoma | breast lobular cancer | |
| cervix adenocarcinoma | clear cell renal cell carcinoma | |
| cervix squamous cell carcinoma | colorectal adenocarcinoma | |
| esophagus adenocarcinoma | oral cavity squamous cell carcinoma | |
| Hodgkin’s lymphoma | ovary endometrioid carcinoma |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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