CEACAM5 Recombinant Antibody AE00357

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE465R to human CEACAM5

Official Symbol: CEACAM5

GeneID: 1048

Swiss-Prot: P06731

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

CEACAM5

All names and symbols: carcinoembryonic antigen-related cell adhesion molecule 5; carcinoembryonic antigen; CD_antigen: CD66e; CEA cell adhesion molecule 5; meconium antigen 100; CEA; CD66e

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2920702

Immunohistochemistry (IHC):

AE00357 to CEACAM5 was successfully used to stain epithelial plasma membranes in human colon, ileum and stomach sections. Recommended concentration: 1-3ug/ml

IHC of colon stained with CEACAM5 Rabbit Recombinant Antibody AE00357
Human colon with AE00357

Formaldehyde-fixed, paraffin-embedded human colon stained with CEACAM5 Rabbit Recombinant Antibody AE00357 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of ileum stained with CEACAM5 Rabbit Recombinant Antibody AE00357
Human ileum with AE00357

Formaldehyde-fixed, paraffin-embedded human ileum stained with CEACAM5 Rabbit Recombinant Antibody AE00357 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of stomach stained with CEACAM5 Rabbit Recombinant Antibody AE00357
Human stomach with AE00357

Formaldehyde-fixed, paraffin-embedded human stomach stained with CEACAM5 Rabbit Recombinant Antibody AE00357 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
appendix mucosaduodenum mucosaadipose
colon mucosasinus paranasalisadrenal gland
ectocervixaorta, media
esophagus squamous epitheliumappendix muscular wall
ileum mucosabone marrow
rectum mucosabreast
stomachbronchus mucosa
tonsilar surface epitheliumcerebellum
cerebrum
colon muscular wall
duodenum Brunner gland
endocervix
endometrium
epididymis
fallopian tube
gallbladder
heart muscle
kidney
liver
lung
lymph node
myometrium
ovarian stroma
pancreas
parathyroid gland
parotid gland
pituitary gland
placenta
pregnant uterus
prostate
seminal vesicle
skeletal muscle
skin
spleen
testis
thymus 
thyroid gland
urinary bladder muscular wall
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
adenocarcinoma of lung and pancreasdiffuse gastric cancerhepatocellular carcinoma
cholangiocarcinomalobular breast cancermalignant mesothelioma
colorectal adenocarcinoma
intestinal type gastric cancer
medullary thyroid cancer
ovarian mucinous carcinoma
squamous cell carcinoma in larinx and cervix
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full-length human CEACAM5 protein

Epitope

Unknown

Cellular localization

cell surface, plasma membrane

Positive Controls

intestine

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