Description
KRT20
All names and symbols: cytokeratin 20; cytokeratin-20; keratin 20; keratin-20; keratin, type I cytoskeletal 20; CK20; CK-20; K20; KRT20
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 92 |
| RRID | AB_2920709 |
Immunohistochemistry (IHC):
AE00363 to KRT20 was successfully used to stain epithelial cells in all human intestinal mucosa, such as in appendix, ileum and colon sections. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human appendix stained with KRT20 Rabbit Recombinant Antibody AE00363 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human ileum stained with KRT20 Rabbit Recombinant Antibody AE00363 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human colon stained with KRT20 Rabbit Recombinant Antibody AE00363 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| appendix mucosa | urothelium | adipose |
| colon mucosa | adrenal gland | |
| duodenum mucosa | aorta, media | |
| ileum mucosa | appendix muscular wall | |
| rectum mucosa | bone marrow | |
| stomach | bronchus mucosa | |
| cerebellum | ||
| cerebrum | ||
| colon muscular wall | ||
| duodenum Brunner gland | ||
| endocervix | ||
| endometrium | ||
| epididymis | ||
| gallbladder | ||
| heart muscle | ||
| kidney | ||
| liver | ||
| lung | ||
| lymph node | ||
| myometrium | ||
| ovarian stroma | ||
| pancreas | ||
| parathyroid gland | ||
| pituitary gland | ||
| seminal vesicle | ||
| skeletal muscle | ||
| spleen | ||
| testis | ||
| thyroid gland | ||
| urinary bladder muscular wall |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| colorectal adenocarcinoma | Merkel cell carcinoma | |
| endometrioid endometrial carcinoma | prostate adenocarcinoma | |
| gastric adenocarcinoma | ||
| ovary mucinous carcinoma |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
Reviews
There are no reviews yet.