KRT20 Recombinant Antibody AE00363

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE670R to human KRT20

Official Symbol: KRT20

GeneID: 54474

Swiss-Prot: P35900

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

KRT20

All names and symbols: cytokeratin 20; cytokeratin-20; keratin 20; keratin-20; keratin, type I cytoskeletal 20; CK20; CK-20; K20; KRT20

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®92
RRIDAB_2920709

Immunohistochemistry (IHC):

AE00363 to KRT20 was successfully used to stain epithelial cells in all human intestinal mucosa, such as in appendix, ileum and colon sections. Recommended concentration: 1-3ug/ml

IHC of appendix stained with KRT20 Rabbit Recombinant Antibody AE00363
Human appendix with AE00363

Formaldehyde-fixed, paraffin-embedded human appendix stained with KRT20 Rabbit Recombinant Antibody AE00363 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of ileum stained with KRT20 Rabbit Recombinant Antibody AE00363
Human ileum with AE00363

Formaldehyde-fixed, paraffin-embedded human ileum stained with KRT20 Rabbit Recombinant Antibody AE00363 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of colon stained with KRT20 Rabbit Recombinant Antibody AE00363
Human colon with AE00363

Formaldehyde-fixed, paraffin-embedded human colon stained with KRT20 Rabbit Recombinant Antibody AE00363 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
appendix mucosaurotheliumadipose
colon mucosaadrenal gland
duodenum mucosaaorta, media
ileum mucosaappendix muscular wall
rectum mucosabone marrow
stomachbronchus mucosa
cerebellum
cerebrum
colon muscular wall
duodenum Brunner gland
endocervix
endometrium
epididymis
gallbladder
heart muscle
kidney
liver
lung
lymph node
myometrium
ovarian stroma
pancreas
parathyroid gland
pituitary gland
seminal vesicle
skeletal muscle
spleen
testis
thyroid gland
urinary bladder muscular wall

Cancer tissues

Strong positiveWeak to moderateNo staining at all
colorectal adenocarcinomaMerkel cell carcinoma
endometrioid endometrial carcinomaprostate adenocarcinoma
gastric adenocarcinoma
ovary mucinous carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment within the aa 196-323 region of human KRT20 protein

Epitope

within aa 196-323 region

Cellular localization

cytoplasm, cytoskeleton

Positive Controls

appendix, colon, ileum

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