INSM1 Recombinant Antibody AE00371

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE456R to human INSM1

Official Symbol: INSM1

GeneID: 3642

Swiss-Prot: Q01101

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

INSM1

All names and symbols: Insulinoma-associated protein 1; INSM transcriptional repressor 1; Zinc finger protein IA-1; INSM1; IA1; IA-1

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®92
RRIDAB_2921272

Immunohistochemistry (IHC):

AE00371 to INSM1 was successfully used to stain nuclei in Islet of Langerhans cells in human pancreas, and neuroendocrine tumour cells in human lung, pancreas, prostate and urinary bladder sections. Recommended concentration: 1-3 μg/ml

IHC of human pancreas stained with INSM1 Rabbit Recombinant Antibody AE00371
Human pancreas with AE00371

Formaldehyde-fixed, paraffin-embedded human pancreas stained with INSM1 Rabbit Recombinant Antibody AE00371 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human pancreas neuroendocrine tumour stained with INSM1 Rabbit Recombinant Antibody AE00371
Human pancreas neuroendocrine tumour with AE00371

Formaldehyde-fixed, paraffin-embedded human pancreas neuroendocrine tumor stained with INSM1 Rabbit Recombinant Antibody AE00371 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human prostate neuroendocrine tumour stained with INSM1 Rabbit Recombinant Antibody AE00371
Human prostate neuroendocrine tumour with AE00371

Formaldehyde-fixed, paraffin-embedded human prostate small cell neuroendocrine tumor stained with INSM1 Rabbit Recombinant Antibody AE00371 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
adrenal gland medullapituitary gland, anterior lobeadipose
pancreasstomachaorta media
thymus appendix mucosa
appendix muscular wall
bone marrow
breast
bronchus mucosa
cerebellum
cerebrum
colon muscular wall
duodenum Brunner gland
duodenum mucosa
ectocervix
endocervix
endometrium
epididymis
esophagus squamous epithelium
fallopian tube
gallbladder
heart muscle
ileum mucosa
kidney
liver
lung
lymph node
myometrium
ovarian stroma
parathyroid gland
parotid gland
pituitary gland posterior lobe
placenta
pregnant uterus
prostate
rectum mucosa
seminal vesicle
sinus paranasalis
skeletal muscle
skin
spleen
testis
thyroid gland
tonsil

Cancer tissues

Low presence of positive erythrocyte signals are ignored

Strong positiveWeak to moderateNo staining at all
ileum neuroendocrine tumourcolorectal adenocarcinomaacinar cell carcinoma
lung neuroendocrine tumourdiffuse large B-cell lymphomaadrenocortical carcinoma
medullary thyroid carcinomaovarian endometrioid carcinomacholangiocarcinoma
Merkel cell carcinomapharynx squamous cell carcinomaclear cell renal cell carcinoma
pancreas neuroendocrine tumourendometrioid endometrial carcinoma
pheochromocytomaesophagus  squamous cell carcinoma
prostate small cell neuroendocrine tumouresophagus adenocarcinoma
urinary bladder small cell neurendocrine tumourgastric adenocarcinoma
gastrointestinal stromal tumor
hepatocellular carcinoma
larynx squamous cell carcinoma
lung adenocarcinoma
malignant melanoma
papillary renal cell carcinoma
papillary thyroid carcinoma
prostate adenocarcinoma
seminoma
thymoma
urothelial carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment of within the aa 81-125 region of human INSM1 protein

Epitope

Within the aa 81-125 region

Cellular localization

nucleus

Positive Controls

neuroendocrine tumors, pancreas

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