MUC1 alpha Recombinant Antibody AE00376

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE672R to human MUC1 subunit alpha

Official Symbol: MUC1

GeneID: 24582

Swiss-Prot: P15941

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

MUC1 subunit alpha

All names and symbols: Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Epithelial membrane antigen; Krebs von den Lungen-6; Mucin-1; Mucin-1 subunit alpha; Peanut-reactive urinary mucin; Polymorphic epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin; ADMCKD; ADMCKD1; ADTKD2; Ca15-3; CA 15-3; CD227; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC1-NT; MUC1-alpha; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; PEM; PEMT; PUM

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2924701

Immunohistochemistry (IHC):

AE00376 to MUC1 alpha subunit was successfully used to stain epithelium of proximal tubuli in healthy kidney, cytoplasm in lung adenocarcinoma, and membranes in lung malignant mesothelioma sections. Recommended concentration: 1-3 μg/ml

IHC of human kidney stained with MUC1 subunit alpha Rabbit Recombinant Antibody AE00376
Human kidney with AE00376

Formaldehyde-fixed, paraffin-embedded human kidney stained with MUC1 subunit alpha Rabbit Recombinant Antibody AE00376 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human lung adenocarcinoma stained with MUC1 subunit alpha Rabbit Recombinant Antibody AE00376
Human lung adenocarcinoma with AE00376

Formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma stained with MUC1 subunit alpha Rabbit Recombinant Antibody AE00376 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human lung malignant mesothelioma stained with MUC1 subunit alpha Rabbit Recombinant Antibody AE00376
Human lung malignant mesothelioma with AE00376

Formaldehyde-fixed, paraffin-embedded human lung malignant mesothelioma stained with MUC1 subunit alpha Rabbit Recombinant Antibody AE00376 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
breastanal skinadipose
bronchus mucosaectocervixadrenal gland
endocervixgallbladderaorta media
endometriumliverappendix mucosa
epididymisparotid glandappendix muscular wall
esophagus squamous epitheliumprostatebone marrow
fallopian tubesinus paranasaliscolon mucosa
kidneystomach colon muscular wall
lungthymusduodenum Brunner gland
pancreasduodenum mucosa
pituitary glandheart muscle
placentaileum mucosa
pregnant uteruslymph node
seminal vesiclemyometrium
skin sebaceous glandovarian stroma
thyroid glandparathyroid gland
tonsilrectum mucosa
urotheliumskeletal muscle

Cancer tissues

Strong positiveWeak to moderateNo staining at all
breast cancer no special typeBrenner Tumoradrenocortical adenoma
cervical squamous cell carcinomagastric adenocarcinomaadrenocortical carcinoma
chromophobe renal cell carcinomapapillary renal cell carcinomacolorectal adenocarcinoma
esophagus  squamous cell carcinomagastrointestinal stromal tumor
esophagus adenocarcinomapancreas neuroendocrine tumor
high-grade serous carcinomaseminoma
lung adenocarcinoma
malignant mesothelioma
pancreas ductal adenocarcinoma
penis squamous cell carcinoma
small cell lung carcinoma
small cell prostate carcinoma
urothelial carcinoma
Warthin tumor
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment of human MUC1 subunit alpha

Epitope

Unknown

Cellular localization

cytoplasm, plasma membrane

Positive Controls

breast, kidney, lung, lung adenocarcinoma, lung malignant mesothelioma

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