Mucin 5AC Recombinant Antibody AE00377

$520,00$1.500,00

Specific, and tissue microarray-validated mouse recombinant antibody clone AE109M to human Mucin 5AC

Official Symbol: MUC5AC

GeneID: 4586

Swiss-Prot: P98088

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

Mucin 5AC

All names and symbols: Gastric mucin; Major airway glycoprotein; mucin 5AC, oligomeric mucus/gel-forming; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin; leB; MUC5; MUC5AC; MUC 5AC; MUC-5AC; TBM

Host speciesMouse
ClonalityRecombinant
IsotypeIgG1, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2924702

Immunohistochemistry (IHC):

AE00377 to Mucin 5AC was successfully used to stain goblet cells in the respiratory epithelium of bronchus, epithelium in stomach, and tumor cells in gastric adenocarcinoma sections. Recommended concentration: 1-3 μg/ml

IHC of human bronchus stained with Mucin 5AC Mouse Recombinant Antibody AE00377
Human bronchus with AE00377

Formaldehyde-fixed, paraffin-embedded human bronchus stained with Mucin 5AC Mouse Recombinant Antibody AE00377 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human stomach stained with Mucin 5AC Mouse Recombinant Antibody AE00377
Human stomach with AE00377

Formaldehyde-fixed, paraffin-embedded human stomach stained with Mucin 5AC Mouse Recombinant Antibody AE00377 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human gastric adenocarcinoma stained with Mucin 5AC Mouse Recombinant Antibody AE00377
Human gastric adenocarcinoma with AE00377

Formaldehyde-fixed, paraffin-embedded human gastric adenocarcinoma stained with Mucin 5AC Mouse Recombinant Antibody AE00377 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
bronchus mucosarectum mucosaadipose
gallbladderadrenal gland
sinus paranasalisaorta media
stomachappendix mucosa
appendix muscular wall
bone marrow
breast
cerebellum
cerebrum
colon mucosa
colon muscular wall
duodenum Brunner gland
duodenum mucosa
ectocervix
endocervix
endometrium, proliferation
endometrium, secretion
epididymis
esophagus squamous epithelium
fallopian tube
heart muscle
ileum mucosa
kidney
liver
lung
lymph node
myometrium
ovarian stroma
pancreas
parathyroid gland
parotid gland
pituitary gland
placenta
prostate
seminal vesicle
skeletal muscle
skin
spleen
testis
thymus
thyroid gland
tonsil
urinary bladder muscular wall

Cancer tissues

Strong positiveWeak to moderateNo staining at all
endometrioid endometrial carcinomacholangiocarcinoma
gastric adenocarcinomahigh-grade serous carcinoma
mucinous carcinomapancreas ductal adenocarcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Epitope

Unknown

Cellular localization

secreted

Positive Controls

bronchus, stomach

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