MSH2 Recombinant Antibody AE00379

$520,00$1.500,00

Specific, and tissue microarray-validated mouse recombinant antibody clone AE902M to human MSH2

Official Symbol: MSH2

GeneID: 4436

Swiss-Prot: P43246

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

MSH2

All names and symbols: DNA mismatch repair protein Msh2; mutS homolog 2; MutS protein homolog 2; COCA1; FCC1; HNPCC; HNPCC1; LCFS2; MMRCS2; MSH-2; hMSH2

Host speciesMouse
ClonalityRecombinant
IsotypeIgG2a, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2924704

Immunohistochemistry (IHC):

AE00379 to MSH2 was successfully used to stain nuclei in normal appendix, esophagus, and in invasive tumor cells of breast sections. Recommended concentration: 1-3 μg/ml

IHC of human appendix stained with MSH2 Mouse Recombinant Antibody AE00379
Human appendix with AE00379

Formaldehyde-fixed, paraffin-embedded human appendix stained with MSH2 Mouse Recombinant Antibody AE00379 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human esophagus stained with MSH2 Mouse Recombinant Antibody AE00379
Human esophagus with AE00379

Formaldehyde-fixed, paraffin-embedded human esophagus stained with MSH2 Mouse Recombinant Antibody AE00379 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human breast carcinoma stained with MSH2 Mouse Recombinant Antibody AE00379
Human breast carcinoma with AE00379

Formaldehyde-fixed, paraffin-embedded human breast carcinoma (no special type) stained with MSH2 Mouse Recombinant Antibody AE00379 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
duodenum Brunner glandendocervixadipose
epididymisadrenal gland
gallbladderaorta media
pancreas ductsappendix mucosa
seminal vesicleappendix muscular wall
stomachbone marrow
breast
bronchus mucosa
cerebellum
cerebrum
colon mucosa
colon muscular wall
duodenum mucosa
ectocervix
endometrium, proliferation
endometrium, secretion
esophagus squamous epithelium
fallopian tube
heart muscle
ileum mucosa
kidney
liver
lung
lymph node
myometrium
ovarian stroma
parathyroid gland
parotid gland
pituitary gland
placenta
prostate
rectum mucosa
skeletal muscle
skin
spleen
testis
thymus
thyroid gland
tonsil
urinary bladder muscular wall
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
breast cancer no special typeesophagus  squamous cell carcinoma
cholangiocarcinomahigh-grade serous carcinoma
colorectal adenocarcinomaMerkel cell carcinoma
endometrioid endometrial carcinomaoral squamous cell carcinoma
esophagus adenocarcinomapapillary renal cell carcinoma
gastric adenocarcinoma diffuse type
gastric adenocarcinoma intestinal type
lung adenocarcinoma
pancreas ductal adenocarcinoma
prostate adenocarcinoma
urothelial carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full-length human MSH2 protein

Epitope

Unknown

Cellular localization

nucleus

Positive Controls

appendix, breast carcinoma, esophagus

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