Nucleolin Recombinant Antibody AE00381

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE623R to human Nucleolin

Official Symbol: NCL

GeneID: 4691

Swiss-Prot: P19338

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

Nucleolin

All names and symbols: Nucleolin; Protein C23; C23; NCL; Nsr1

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2924901

Immunohistochemistry (IHC):

AE00381 to Nucleolin was successfully used to stain nuclei with emphasis on the nucleoli in normal fallopian tube and gallbladder and in urothelial carcinoma sections. Recommended concentration: 1-3 μg/ml

IHC of human fallopian tube stained with Nucleolin Rabbit Recombinant Antibody AE00381
Human fallopian tube with AE00381

Formaldehyde-fixed, paraffin-embedded human fallopian tube stained with Nucleolin Rabbit Recombinant Antibody AE00381 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human gallbladder stained with Nucleolin Rabbit Recombinant Antibody AE00381
Human gallbladder with AE00381

Formaldehyde-fixed, paraffin-embedded human gallbladder stained with Nucleolin Rabbit Recombinant Antibody AE00381 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human urothelial carcinoma stained with Nucleolin Rabbit Recombinant Antibody AE00381
Human urothelial carcinoma with AE00381

Formaldehyde-fixed, paraffin-embedded human urothelial carcinoma stained with Nucleolin Rabbit Recombinant Antibody AE00381 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
adrenal glandcerebrum grey matteradipose
appendix mucosamyometriumaorta media
appendix muscular wallpituitary gland posterior lobebone marrow
breastheart muscle
bronchus mucosa
cerebellum
cerebrum white matter
colon mucosa
colon muscular wall
duodenum Brunner gland
duodenum mucosa
endocervix
ectocervix
endometrium, proliferation
endometrium, secretion
epididymis
esophagus squamous epithelium
fallopian tube
gallbladder
ileum mucosa
liver
lung
lymph node
ovarian stroma
pancreas
parathyroid gland
parotid gland
pituitary gland anterior lobe
placenta
prostate
rectum mucosa
renal cortex
renal medulla
seminal vesicle
sinus paranasalis
skeletal muscle
skin
spleen
stomach
testis
thymus
thyroid gland
tonsil
urinary bladder muscular wall
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
breast cancer no special type
cervix adenocarcinoma
cervix squamous cell carcinoma
clear cell ovarian carcinoma
clear cell renal cell carcinoma
colorectal adenocarcinoma
endometrial serous adenocarcinoma
endometrioid endometrial carcinoma
esophagus  squamous cell carcinoma
esophagus adenocarcinoma
follicular thyroid carcinoma
high-grade serous carcinoma
invasive lobular carcinoma
mucinous ovarian carcinoma
oral squamous cell carcinoma
ovarian endometrioid carcinoma
papillary renal cell carcinoma
papillary thyroid carcinoma
pharynx squamous cell carcinoma
prostate adenocarcinoma
renal oncocytoma
urothelial carcinoma muscle invasive
urothelial carcinoma papillary non-invasive
vulva squamous cell carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full-length human Nucleolin protein

Epitope

Unknown

Cellular localization

nucleolus, nucleus

Positive Controls

fallopian tube, gallbladder, urothelial carcinoma

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