TP16 Recombinant Antibody AE00382

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE016R to human TP16

Official Symbol: CDKN2A

GeneID: 1029

Swiss-Prot: P42771

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

TP16

All names and symbols: Cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; Melanoma, cutaneous malignant 2; Multiple tumor suppressor 1; CDK4I; CDKN2; CDKN2A; CMM2; MTS1; MTS-1; p16; p16-INK4; p16-INK4A; p16INK4; p16INK4A; TP16

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2924902

Immunohistochemistry (IHC):

AE00382 to TP16 was successfully used to stain nuclei in the pituitary gland anterior lobe, and cytoplasm and nuclei in thyroid carcinoma and urothelial carcinoma sections. Recommended concentration: 1-3 μg/ml

IHC of human pituitary gland stained with TP16 Rabbit Recombinant Antibody AE00382
Human pituitary gland with AE00382

Formaldehyde-fixed, paraffin-embedded human pituitary gland anterior lobe stained with TP16 Rabbit Recombinant Antibody AE00382 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human thyroid carcinoma stained with TP16 Rabbit Recombinant Antibody AE00382
Human thyroid carcinoma with AE00382

Formaldehyde-fixed, paraffin-embedded human anaplastic thyroid carcinoma stained with TP16 Rabbit Recombinant Antibody AE00382 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human urothelial carcinoma stained with TP16 Rabbit Recombinant Antibody AE00382
Human urothelial carcinoma with AE00382

Formaldehyde-fixed, paraffin-embedded human muscle-invasive urothelial carcinoma stained with TP16 Rabbit Recombinant Antibody AE00382 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
pituitary gland anterior lobeadrenal glandadipose
appendix mucosaappendix muscular wall
breastaorta media
duodenum mucosabronchus mucosa
endometrium, proliferationcerebellum
endometrium, secretioncerebrum
pancreascolon mucosa
prostatecolon muscular wall
sinus paranasalisduodenum Brunner gland
thymus endocervix
tonsilectocervix
urotheliumepididymis
esophagus squamous epithelium
fallopian tube
gallbladder
heart muscle
ileum mucosa
liver
lung
lymph node
myometrium
ovarian stroma
parathyroid gland
parotid gland
placenta
rectum mucosa
renal cortex
renal medulla
seminal vesicle
skeletal muscle
skin
spleen
stomach
testis
thyroid gland
urinary bladder muscular wall

Cancer tissues

Strong positiveWeak to moderateNo staining at all
anaplastic thyroid carcinoma
cervix squamous cell carcinoma
high-grade serous carcinoma
leiomyosarcoma
liposarcoma
melanoma
Merkel cell carcinoma
oral squamous cell carcinoma
urinary bladder small cell carcinoma
urothelial carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full-length human TP16 protein

Epitope

Unknown

Cellular localization

cytoplasm, nucleus

Positive Controls

anaplastic thyroid carcinoma, pituitary gland, urothelial carcinoma

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