Description
TP53
All names and symbols: Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor protein p53; Tumor suppressor p53; BCC7; LFS1; P53; TRP53
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 90 |
| RRID | AB_ |
Immunohistochemistry (IHC):
AE00383 to TP53 was successfully used to stain nuclei colorectal adenocarcinoma, esophagus squamous cell carcinoma and high-grade serous carcinoma sections. Recommended concentration: 1-3 μg/ml
Formaldehyde-fixed, paraffin-embedded human colorectal adenocarcinoma stained with TP53 Rabbit Recombinant Antibody AE00383 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human esophagus squamous cell carcinoma stained with TP53 Rabbit Recombinant Antibody AE00383 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human high-grade serous carcinoma stained with TP53 Rabbit Recombinant Antibody AE00383 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| endometrium, proliferation | adipose | |
| epididymis | adrenal gland | |
| esophagus squamous epithelium | aorta media | |
| gallbladder | appendix mucosa | |
| ileum mucosa | appendix muscular wall | |
| lymph node | bone marrow | |
| ovary corpus luteum | breast | |
| prostate | bronchus mucosa | |
| renal cortex | cerebellum | |
| skin | cerebrum | |
| tonsil | colon mucosa | |
| urothelium | colon muscular wall | |
| duodenum Brunner gland | ||
| duodenum mucosa | ||
| ectocervix | ||
| endocervix | ||
| endometrium, secretion | ||
| fallopian tube | ||
| heart muscle | ||
| liver | ||
| lung | ||
| myometrium | ||
| ovarian stroma | ||
| pancreas | ||
| parathyroid gland | ||
| parotid gland | ||
| pituitary gland both lobes | ||
| placenta | ||
| rectum mucosa | ||
| renal medulla | ||
| seminal vesicle | ||
| sinus paranasalis | ||
| skeletal muscle | ||
| spleen | ||
| stomach | ||
| testis | ||
| thymus | ||
| thyroid gland | ||
| urinary bladder muscular wall |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| colorectal adenocarcinoma | clear cell ovarian carcinoma | clear cell renal cell carcinoma |
| esophagus squamous cell carcinoma | endometrioid ovarian carcinoma | invasive lobular carcinoma |
| high-grade serous carcinoma | papillary renal cell carcinoma | papillary thyroid carcinoma |
| urothelial carcinoma | prostate adenocarcinoma | |
| renal oncocytoma |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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