EGFR-pY1068 Polyclonal Antibody AE00210

520,00825,00

Phospho-specific rabbit polyclonal antibody to human EGFR, phosphorylated Y1068 (EGFR-pY1068)

See all our EGFR antibodies

Official Symbol: EGFR

GeneID: 1956

Swiss-Prot: P00533

Description

EGFR-pY1068

All names and symbols: Epithelial Growth Factor Receptor; EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIP, WB
Aeonian Rating®70
RRIDAB_2813764

Immunoprecipitation (IP):

AE00210 to EGFR-pY1068 was successfully used to immunoprecipitate an approx. 180kDa band from lysates of EGF-stimulated A431. Recommended amount: 3-6ug

IP from A431, WB stained with EGFR-pY1068 Rabbit Phospho-Specific Polyclonal Antibody AE00210
IP from A431 with AE00210

Western Blot of immunoprecipitation (lanes 2 and 3) from 200ug A431 (30 min stimulation by 100ng/ml EGF after overnight serum-starvation) extract, using 3ug EGFR-pY1068 Rabbit Polyclonal Antibody AE00210, and using the same antibody for staining the blot at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Western Blot (WB):

AE00210 to EGFR-pY1068 was successfully used to stain an approx. 180kDa band in lysates of HeLa after overnight serum-starvation and subsequent treatment with either EGF or ATP. Recommended concentration: 1-3ug/ml

WB of HeLa stained with EGFR-pY1068 Rabbit Phospho-Specific Polyclonal Antibody AE00210
HeLa with AE00210

Western Blot of 30ug lysate from serum-starved HeLa with or without stimulation by 100mg/ml EGF (30 min, 37C) or by 5mM ATP (1h, 30C), stained with EGFR-pY1068 Rabbit Polyclonal Antibody AE00210 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated Y1092 of human EGFR (NP_005219.2) corresponding to Y1068 of the mature protein (starts with L25).

Epitope

A short sequence around phosphorylated Y1068 (exact sequence is proprietary)

Cellular localization

cell surface, plasma membrane, translocation to nucleus upon EGF stimulation

Positive Controls

ATP-treated serum-starved HeLa, EGF-stimulated serum-starved A431

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