Description
EGFR-pY1068
All names and symbols: Epithelial Growth Factor Receptor; EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Confirmed species reactivity | Human, Mouse, Rat |
| Confirmed applications | ICC, IHC, WB |
| Aeonian Rating® | 70 |
| RRID | AB_2892794 |
Immunocytochemistry (ICC):
AE00304 to EGFR-pY1068 was successfully used to stain nuclear membranes in LPS-treated cell line HepG2. Recommended concentration: 5-15ug/ml
Confocal microscopy of PFA-fixed, and LPS-treated for 4h, HepG2 (0.1% TX100 permeabilised) stained with EGFR-pY1068 Rabbit Polyclonal Antibody AE00304 at 5ug/ml (1h at 37℃). Detection by AF594 (red) for the EGFR-pY1068 rabbit antibody, AF488 (green) for beta-tubulin mouse antibody, and DAPI (blue) for nuclear staining.
Immunohistochemistry (IHC):
AE00304 to EGFR-pY1068 was successfully used to stain nuclei in human colon carcinoma sections . Recommended concentration: 5-15ug/ml
Formaldehyde-fixed, paraffin-embedded human colorectal carcinoma stained with EGFR-pY1068 Rabbit Polyclonal Antibody AE00304 at 10ug/ml 1.5h at ambient temperature. Epitope retrieval: Pressure cooker heating at pH6 for 3min, then cool by running cold water for 10min. DAB staining by HRP.
Western Blot (WB):
AE00304 to EGFR-pY1068 was successfully used to stain an approx. 160kDa band in mouse and rat liver lysates. Recommended concentration: 1-3ug/ml
Western Blot of 10ug liver lysates from mouse (lanes 1 and 2) and rat (lanes 3 and 4) stained with EGFR-pY1068 Rabbit Polyclonal Antibody AE00304 at 1ug/ml (overnight at 4℃) in the presence (+) or absence (-) of its antigen (P peptide) or its non-phosphorylated control (C peptide). ECL staining by HRP.
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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