CD8a Recombinant Antibody AE00353

520,001.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE008R to human CD8a

See all our CD8a antibodies

Official Symbol: CD8A

GeneID: 925

Swiss-Prot: P01732

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

CD8a

All names and symbols: CD8a molecule; CD_antigen: CD8a; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8; Leu2; MAL; p32

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2916357

Immunohistochemistry (IHC):

AE00353 to CD8a was successfully used to stain plasma membranes of T-cells in human duodenum, lymph node, and thymus sections. Recommended concentration: 1-3ug/ml

IHC of human duodenum stained with CD8a Rabbit Recombinant Antibody AE00353
Human duodenum with AE00353

Formaldehyde-fixed, paraffin-embedded human duodenum stained with CD8a Rabbit Recombinant Antibody AE00353 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human lymph node stained with CD8a Rabbit Recombinant Antibody AE00353
Human lymph node with AE00353

Formaldehyde-fixed, paraffin-embedded human lymph node stained with CD8a Rabbit Recombinant Antibody AE00353 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human thymus stained with CD8a Rabbit Recombinant Antibody AE00353
Human thymus with AE00353

Formaldehyde-fixed, paraffin-embedded human thymus stained with CD8a Rabbit Recombinant Antibody AE00353 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
lymph nodeadrenal glandadipose
spleenappendix mucosaaorta, media
thymusbronchus mucosaappendix muscular wall
tonsilcolon mucosabone marrow
colon muscular wallbreast
duodenum mucosacerebellum
ectocervixcerebrum
endocervixduodenum Brunner gland
endometrium, proliferatoryendometrium, pregnancy
epididymisendometrium, secretory
esophagus squamous epitheliumheart muscle
fallopian tubekidney
gallbladdermyometrium
ileum mucosaovarian stroma
liverparathyroid gland
lungpituitary gland
pancreasplacenta
parotid glandprostate
rectum mucosaskeletal muscle
seminal vesicleskin
sinus paranasalistestis
stomachthyroid gland
urotheliumurinary bladder muscular wall

Cancer tissues

Strong positiveWeak to moderateNo staining at all
angioimmunoblastic T-cell lymphomachronic lymphocytic leukemia in lymph nodepapillary renal cell carcinoma
cervix squamous cell carcinomaclear cell renal cell carcinoma
follicular lymphoma in lymph nodeesophagus adenocarcinoma
Hodgkin’s lymphomahigh grade serous carcinoma
vagina squamous cell carcinomaprostate adenocarcinoma
thyroid follicular carcinoma
uruthelial carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Synthetic peptide corresponding to a region within aa 135-235 of human CD8a protein

Epitope

Unknown

Cellular localization

cell surface, plasma membrane

Positive Controls

duodenum, lymph node, spleen, thymus, tonsil

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