CD45 Recombinant Antibody AE00356

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE045R to human CD45

Official Symbol: CD45

GeneID: 5788

Swiss-Prot: P08575

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

CD45

All names and symbols: CD_antigen: CD45; Leukocyte common antigen; protein tyrosine phosphatase receptor type C; Receptor-type tyrosine-protein phosphatase C; B220; CD45; L-CA; T200; CD45R; GP180; LCA; LY5; PTPRC; T200

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2920700

Immunohistochemistry (IHC):

AE00356 to CD45 was successfully used to stain lymphocyte plasma membranes in human lymph node, spleen and tonsil sections. Recommended concentration: 1-3ug/ml

IHC of human lymph node stained with CD45 Rabbit Recombinant Antibody AE00356
Human lymph node with AE00356

Formaldehyde-fixed, paraffin-embedded human lymph node stained with CD45 Rabbit Recombinant Antibody AE00356 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of spleen stained with CD45 Rabbit Recombinant Antibody AE00356
Human spleen with AE00356

Formaldehyde-fixed, paraffin-embedded human spleen stained with CD45 Rabbit Recombinant Antibody AE00356 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of tonsil stained with CD45 Rabbit Recombinant Antibody AE00356
Human tonsil with AE00356

Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD45 Rabbit Recombinant Antibody AE00356 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
appendix mucosaadrenal glandadipose
duodenum mucosaappendix muscular wallaorta, media
ileum mucosabone marrowcerebellum
liverbronchus mucosacolon muscular wall
lymph nodecerebrumheart muscle
rectum mucosaduodenum Brunner glandovarian stroma
spleenendocervixparathyroid gland
thymusendometriumplacenta
tonsilesophageal squamous epitheliumseminal vesicle
fallopian tubetestis
gallbladderthyroid gland
kidney
lung
myometrium
pancreas
parotid gland
pituitary gland, posterior lobe
prostate
sinus paranasalis
skeletal muscle
skin
stomach
urinary bladder muscular wall
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
ALK+ anaplastic large cell lymphomacolorectal adenocarcinomaanaplastic large cell lymphoma
angioimmunoblastic T-cell lymphomadiffuse large B-cell lymphomaclear cell ovarian carcinoma
chronic lymphocytic leukemia in lymph nodehigh-grade serous carcinoma
follicular lymphomavaginal squamous cell carcinoma
T-lymphoblastic lymphoma
mantle cell lymphoma
marginal zone lymphoma
oropharyngeal squamous cell carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full-length human CD45 protein

Epitope

Unknown

Cellular localization

cell surface, plasma membrane

Positive Controls

intestine, lymph node, thymus, tonsil

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