KRT5 Recombinant Antibody AE00361

$520,00$1.500,00

Specific, and tissue microarray-validated mouse recombinant antibody clone AE605M to human KRT5

Official Symbol: KRT5

GeneID: 3852

Swiss-Prot: P13647

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

KRT5

All names and symbols: cytokeratin 5; cytokeratin-5; keratin 5; Keratin-5; Keratin, type II cytoskeletal 5; Type-II keratin Kb5; K5; CK5; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; KRT5; KRT5A

Host speciesMouse
ClonalityRecombinant
IsotypeIgG2a, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®91
RRIDAB_2920707

Immunohistochemistry (IHC):

AE00361 to KRT5 was successfully used to stain basal cells in human epididymis, skin and urothelium sections. Recommended concentration: 1-3ug/ml

IHC of epididymis stained with KRT5 Mouse Recombinant Antibody AE00361
Human epididymis with AE00361

Formaldehyde-fixed, paraffin-embedded human epididymis stained with KRT5 Mouse Recombinant Antibody AE00361 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of skin stained with KRT5 Mouse Recombinant Antibody AE00361
Human skin with AE00361

Formaldehyde-fixed, paraffin-embedded human skin stained with KRT5 Mouse Recombinant Antibody AE00361 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of urothelium stained with KRT5 Mouse Recombinant Antibody AE00361
Human urothelium with AE00361

Formaldehyde-fixed, paraffin-embedded human urothelium stained with KRT5 Mouse Recombinant Antibody AE00361 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
bronchus mucosaendocervical glandsadipose
ectocervixparotid glandadrenal gland
epididymisthymusaorta, media
esophagus squamous epitheliumappendix mucosa
prostateappendix muscular wall
sinus paranasalisbone marrow
skinbreast
tonsilcerebellum
urotheliumcerebrum
colon mucosa
duodenum Brunner gland
duodenum mucosa
endometrium
gallbladder
heart muscle
kidney
liver
lung
ovarian stroma
pancreas
parathyroid gland
pituitary gland
placenta
pregnant uterus
rectum mucosa
seminal vesicle
spleen
stomach
testis
thyroid gland
urinary bladder muscular wall

Cancer tissues

Strong positiveWeak to moderateNo staining at all
breast cancer no special typecholangiocarcinoma
cervical squamous cell carcinomalobular breast cancer
esophagus squamous cell carcinomamalignant mesethelioma
lung squamous cell carcinoma
pancreas adenocarcinoma
thymoma
urothelial carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment within the aa 316-590 region of human KRT5 protein

Epitope

within aa 316-590 region

Cellular localization

cytoskeleton

Positive Controls

epididymis, skin, urothelium

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