Decorin Recombinant Antibody AE00364

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE537R to human Decorin

Official Symbol: DCN

GeneID: 1634

Swiss-Prot: P07585

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

Decorin

All names and symbols: bone proteoglycan II; decorin; CSCD; DCN; DSPG2; PG40; PG-40; PG-S2; PGII; PGS2; SLRR1B

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®92
RRIDAB_2920710

Immunohistochemistry (IHC):

AE00364 to Decorin was successfully used to stain human muscle tissues, and stroma in glands and intestines, such as in colon muscular wall, epididymis, and gastric mucosa sections. Recommended concentration: 1-3ug/ml

IHC of colon muscular wall stained with KRT20 Rabbit Recombinant Antibody AE00364
Human colon muscular wall with AE00364

Formaldehyde-fixed, paraffin-embedded human colon muscular wall stained with Decorin Rabbit Recombinant Antibody AE00364 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of epididymis stained with KRT20 Rabbit Recombinant Antibody AE00364
Human epididymis wall with AE00364

Formaldehyde-fixed, paraffin-embedded human epididymis stained with Decorin Rabbit Recombinant Antibody AE00364 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of stomach stained with KRT20 Rabbit Recombinant Antibody AE00364
Human stomach with AE00364

Formaldehyde-fixed, paraffin-embedded human gastric mucosa stained with Decorin Rabbit Recombinant Antibody AE00364 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
appendix mucosaadrenal glandadipose
appendix muscular wallcolon mucosaaorta, media
breastduodenum mucosabone marrow
bronchus mucosaendometriumcerebellum
colon muscular wallgallbladdercerebrum
duodenum Brunner glandileum mucosakidney
ectocervixpancreasliver
endocervixparathyroid glandlymph node
endometrium, pregnancyparotid glandspleen
epididymispituitary gland, anterior lobethymus
esophagus squamous epitheliumtonsil
fallopian tube
heart muscle
myometrium
ovarian stroma
placenta
prostate
rectum mucosa
seminal vesicle
sinus paranasalis
skin
stomach
testis
thyroid gland
urinary bladder muscular wall
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
anal squamous cell carcinomabreast cancer no special typehepatocellular carcinoma
colorectal adenocarcinomahigh-grade serous carcinomaHodgkin’s lymphoma
esophagus adenocarcinomaMerkel cell carcinoma
leiomyosarcomaWarthin tumor
liposarcoma
pancreas neuroendocrine tumor
pancreatic ductal adenocarcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment within the aa 212-336 region of human Decorin protein

Epitope

within aa 212-336 region

Cellular localization

extracellular space, secreted

Positive Controls

glands, intestine, skeletal muscle, smooth muscle cells

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