Description
Decorin
All names and symbols: bone proteoglycan II; decorin; CSCD; DCN; DSPG2; PG40; PG-40; PG-S2; PGII; PGS2; SLRR1B
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 92 |
| RRID | AB_2920710 |
Immunohistochemistry (IHC):
AE00364 to Decorin was successfully used to stain human muscle tissues, and stroma in glands and intestines, such as in colon muscular wall, epididymis, and gastric mucosa sections. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human colon muscular wall stained with Decorin Rabbit Recombinant Antibody AE00364 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human epididymis stained with Decorin Rabbit Recombinant Antibody AE00364 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human gastric mucosa stained with Decorin Rabbit Recombinant Antibody AE00364 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| appendix mucosa | adrenal gland | adipose |
| appendix muscular wall | colon mucosa | aorta, media |
| breast | duodenum mucosa | bone marrow |
| bronchus mucosa | endometrium | cerebellum |
| colon muscular wall | gallbladder | cerebrum |
| duodenum Brunner gland | ileum mucosa | kidney |
| ectocervix | pancreas | liver |
| endocervix | parathyroid gland | lymph node |
| endometrium, pregnancy | parotid gland | spleen |
| epididymis | pituitary gland, anterior lobe | thymus |
| esophagus squamous epithelium | tonsil | |
| fallopian tube | ||
| heart muscle | ||
| myometrium | ||
| ovarian stroma | ||
| placenta | ||
| prostate | ||
| rectum mucosa | ||
| seminal vesicle | ||
| sinus paranasalis | ||
| skin | ||
| stomach | ||
| testis | ||
| thyroid gland | ||
| urinary bladder muscular wall | ||
| urothelium |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| anal squamous cell carcinoma | breast cancer no special type | hepatocellular carcinoma |
| colorectal adenocarcinoma | high-grade serous carcinoma | Hodgkin’s lymphoma |
| esophagus adenocarcinoma | Merkel cell carcinoma | |
| leiomyosarcoma | Warthin tumor | |
| liposarcoma | ||
| pancreas neuroendocrine tumor | ||
| pancreatic ductal adenocarcinoma |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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