Description
ESR1
All names and symbols: Estrogen receptor 1; Estrogen receptor-1; Estrogen receptor alpha; Estrogen receptor-alpha; ERalpha; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ER; ESR; ESRA; ESTRR; Era; NR3A1
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 93 |
| RRID | AB_2920806 |
Immunohistochemistry (IHC):
AE00368 to ESR1 was successfully used to stain nuclei in epithelial cells of human epididymis, fallopian tube and endometrium sections. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human epididymis stained with ESR1 Rabbit Recombinant Antibody AE00368 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human fallopian tube stained with ESR1 Rabbit Recombinant Antibody AE00368 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human endometrium, secretory phase stained with ESR1 Rabbit Recombinant Antibody AE00368 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| ectocervix | parotid gland | adipose |
| endocervix | pituitary gland anterior lobe | adrenal gland |
| endometrium | urothelium | aorta, media |
| epididymis, cauda | appendix mucosa | |
| fallopian tube | appendix muscular wall | |
| myometrium | bone marrow | |
| breast | ||
| bronchus mucosa | ||
| cerebellum | ||
| cerebrum | ||
| colon mucosa | ||
| colon muscular wall | ||
| duodenum Brunner gland | ||
| duodenum mucosa | ||
| epididymis, corpus | ||
| esophagus squamous epithelium | ||
| gallbladder | ||
| heart muscle | ||
| ileum mucosa | ||
| kidney | ||
| lung | ||
| lymph node | ||
| ovarian stroma | ||
| pancreas | ||
| parathyroid gland | ||
| pituitary gland posterior lobe | ||
| placenta | ||
| prostate | ||
| rectum mucosa | ||
| seminal vesicle | ||
| sinus paranasalis | ||
| skeletal muscle | ||
| skin | ||
| spleen | ||
| stomach | ||
| testis | ||
| thymus | ||
| thyroid gland | ||
| tonsil | ||
| urinary bladder muscular wall |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| breast cancer no special type | clear cell renal cell carcinoma | |
| endometrioid ovarian carcinoma | colorectal adenocarcinoma | |
| high-grade serous carcinoma | esophagus adenocarcinoma | |
| oral squamous cell carcinoma | ||
| papillary renal cell carcinoma |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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