Inhibin alpha Recombinant Antibody AE00370

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE561R to human Inhibin alpha

Official Symbol: INHA

GeneID: 3623

Swiss-Prot: P05111

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

Inhibin alpha

All names and symbols: Inhibin A; inhibin alpha chain; inhibin alpha subunit; inhibin subunit alpha; INHA; INH-A

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2920808

Immunohistochemistry (IHC):

AE00370 to Inhibin alpha was successfully used to stain advanced follicular granulosa cells in human ovary, Sertoli and Leydig cells in human testis, and trophoblasts in human placenta sections. Recommended concentration: 1-3ug/ml

IHC of human ovary stained with Inhibin alpha Rabbit Recombinant Antibody AE00370
Human ovary with AE00370

Formaldehyde-fixed, paraffin-embedded human ovary stained with Inhibin alpha Rabbit Recombinant Antibody AE00370 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human testis stained with Inhibin alpha Rabbit Recombinant Antibody AE00370
Human testis with AE00370

Formaldehyde-fixed, paraffin-embedded human testis stained with Inhibin alpha Rabbit Recombinant Antibody AE00370 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human placenta stained with Inhibin alpha Rabbit Recombinant Antibody AE00370
Human placenta with AE00370

Formaldehyde-fixed, paraffin-embedded human placenta stained with Inhibin alpha Rabbit Recombinant Antibody AE00370 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
adrenal glandovary primary folliclesadipose
ovary advanced folliclesovarian stromaaorta, media
placenta, earlyendometrium, pregnancyappendix mucosa
testisplacenta, matureappendix muscular wall
bone marrow
breast
bronchus mucosa
cerebellum
cerebrum
colon mucosa
colon muscular wall
duodenum Brunner gland
duodenum mucosa
ectocervix
endocervix
endometrium
epididymis
esophagus squamous epithelium
fallopian tube
gallbladder
heart muscle
heart muscle
kidney
liver
lung
lymph node
myometrium
parathyroid gland
parotid gland
pituitary gland
prostate
rectum mucosa
seminal vesicle
sinus paranasalis
skeletal muscle
skin
spleen
stomach
thymus 
thyroid gland
tonsil
urothelium

Cancer tissues

Low presence of positive erythrocyte signals are ignored

Strong positiveWeak to moderateNo staining at all
adrenal cortical adenomacholangiocarcinomabreast cancer no special type
adrenocortical carcinomacolorectal adenocarcinomaesophagus  squamous cell carcinoma
lung adenocarcinomagastric adenocarcinoma
pancreas acinar cell carcinomaMerkel cell carcinoma
pancreas neuroendocrine tumorpancreas adenocarcinoma
papillary thyroid carcinoma
prostate adenocarcinoma
seminosa
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment of within the aa 233-362 region of human Inhibin alpha protein

Epitope

Within the aa 233-362 region (mature alpha chain)

Cellular localization

extracellular space, secreted

Positive Controls

ovary, placenta, testis

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