Description
Mucin 5AC
All names and symbols: Gastric mucin; Major airway glycoprotein; mucin 5AC, oligomeric mucus/gel-forming; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin; leB; MUC5; MUC5AC; MUC 5AC; MUC-5AC; TBM
| Host species | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, kappa |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 90 |
| RRID | AB_2924702 |
Immunohistochemistry (IHC):
AE00377 to Mucin 5AC was successfully used to stain goblet cells in the respiratory epithelium of bronchus, epithelium in stomach, and tumor cells in gastric adenocarcinoma sections. Recommended concentration: 1-3 μg/ml
Formaldehyde-fixed, paraffin-embedded human bronchus stained with Mucin 5AC Mouse Recombinant Antibody AE00377 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human stomach stained with Mucin 5AC Mouse Recombinant Antibody AE00377 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human gastric adenocarcinoma stained with Mucin 5AC Mouse Recombinant Antibody AE00377 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| bronchus mucosa | rectum mucosa | adipose |
| gallbladder | adrenal gland | |
| sinus paranasalis | aorta media | |
| stomach | appendix mucosa | |
| appendix muscular wall | ||
| bone marrow | ||
| breast | ||
| cerebellum | ||
| cerebrum | ||
| colon mucosa | ||
| colon muscular wall | ||
| duodenum Brunner gland | ||
| duodenum mucosa | ||
| ectocervix | ||
| endocervix | ||
| endometrium, proliferation | ||
| endometrium, secretion | ||
| epididymis | ||
| esophagus squamous epithelium | ||
| fallopian tube | ||
| heart muscle | ||
| ileum mucosa | ||
| kidney | ||
| liver | ||
| lung | ||
| lymph node | ||
| myometrium | ||
| ovarian stroma | ||
| pancreas | ||
| parathyroid gland | ||
| parotid gland | ||
| pituitary gland | ||
| placenta | ||
| prostate | ||
| seminal vesicle | ||
| skeletal muscle | ||
| skin | ||
| spleen | ||
| testis | ||
| thymus | ||
| thyroid gland | ||
| tonsil | ||
| urinary bladder muscular wall |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| endometrioid endometrial carcinoma | cholangiocarcinoma | |
| gastric adenocarcinoma | high-grade serous carcinoma | |
| mucinous carcinoma | pancreas ductal adenocarcinoma |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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