Description
MSH6
All names and symbols: DNA mismatch repair protein Msh6; G/T mismatch-binding protein ; MutS-alpha 160 kDa subunit (p160); MutS protein homolog 6; GTBP; GTMBP; HNPCC5; HSAP; hMSH6; MSH-6; MMRCS3; p160
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 92 |
| RRID | AB_2924705 |
Immunohistochemistry (IHC):
AE00380 to MSH6 was successfully used to stain nuclei in normal esophagus and skin sections, and in ovarian endometriod carcinoma sections. Recommended concentration: 1-3 μg/ml
Formaldehyde-fixed, paraffin-embedded human esophagus stained with MSH6 Rabbit Recombinant Antibody AE00380 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human skin stained with MSH6 Rabbit Recombinant Antibody AE00380 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human ovarian endometrioid carcinoma stained with MSH6 Rabbit Recombinant Antibody AE00380 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| adrenal gland | appendix muscular wall | adipose |
| appendix mucosa | cerebellum | aorta media |
| bone marrow | cerebrum | |
| breast | colon muscular wall | |
| bronchus mucosa | gallbladder | |
| colon mucosa | heart muscle | |
| duodenum Brunner gland | lung | |
| duodenum mucosa | pituitary gland posterior lobe | |
| endocervix | skeletal muscle | |
| ectocervix | urinary bladder muscular wall | |
| endometrium, proliferation | ||
| endometrium, secretion | ||
| epididymis | ||
| esophagus squamous epithelium | ||
| fallopian tube | ||
| ileum mucosa | ||
| liver | ||
| lymph node | ||
| myometrium | ||
| ovarian stroma | ||
| pancreas | ||
| parathyroid gland | ||
| parotid gland | ||
| pituitary gland anterior lobe | ||
| placenta | ||
| prostate | ||
| rectum mucosa | ||
| renal cortex | ||
| renal medulla | ||
| seminal vesicle | ||
| sinus paranasalis | ||
| skin | ||
| spleen | ||
| stomach | ||
| testis | ||
| thymus | ||
| thyroid gland | ||
| tonsil | ||
| urothelium |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| breast cancer no special type | clear cell ovarian carcinoma | |
| colorectal adenocarcinoma | pancreas ductal adenocarcinoma | |
| gastric adenocarcinoma | prostate adenocarcinoma | |
| high-grade serous carcinoma | ||
| lung adenocarcinoma | ||
| Mullerian ovary tumor | ||
| Mullerian uterus tumor | ||
| ovarian endometrioid carcinoma | ||
| pancreas acinar cell carcinoma |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
Reviews
There are no reviews yet.