TP63 Recombinant Antibody AE00384

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE063R to human TP63

Official Symbol: TP63

GeneID: 8626

Swiss-Prot: Q9H3D4

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

TP63

All names and symbols: Chronic ulcerative stomatitis protein; Keratinocyte transcription factor KET; Transformation-related protein 63; Tumor protein p63; Tumor protein p73-like; AIS; B(p51A); B(p51B); CUSP; EEC3; KET; LMS; NBP; OFC8; p40; p51; p53CP; p63; p73L; p73H; p73L; RHS; SHFM4; TP53CP; TP53L; TP63; TP73L

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®92
RRIDAB_2924904

Immunohistochemistry (IHC):

AE00384 to TP63 was successfully used to stain nuclei in sections of epididymis, esophagus and squamous cell carcinoma of the esophagus and of the pharynx. Recommended concentration: 1-3 μg/ml

IHC of human epididymis stained with TP63 Rabbit Recombinant Antibody AE00384
Human epididymis with AE00384

Formaldehyde-fixed, paraffin-embedded human epididymis stained with TP63 Rabbit Recombinant Antibody AE00384 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human esophagus and its squamous cell carcimona stained with TP63 Rabbit Recombinant Antibody AE00384
Human esophagus /squamous cell carcinoma with AE00384

Formaldehyde-fixed, paraffin-embedded human esophagus (left) and esophagus squamous cell carcinoma (right) stained with TP63 Rabbit Recombinant Antibody AE00384 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human pharynx squamous cell carcimona stained with TP63 Rabbit Recombinant Antibody AE00384
Human pharynx squamous cell carcinoma with AE00384

Formaldehyde-fixed, paraffin-embedded human pharynx squamous cell carcinoma stained with TP63 Rabbit Recombinant Antibody AE00384 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
breastappendix mucosaadipose
bronchus mucosalymph nodeadrenal gland
ectocervixaorta media
epididymisappendix muscular wall
esophagus squamous epitheliumbone marrow
parotid glandcerebellum
placenta earlycerebrum
placenta maturecolon mucosa
prostatecolon muscular wall
renal pelvisduodenum Brunner gland
seminal vesicleduodenum mucosa
skin endocervix
skin sebaceous glandsendometrium, proliferation
thymus endometrium, secretion
tonsilfallopian tube
heart muscle
ileum mucosa
liver
lung
myometrium
ovarian stroma
pancreas
parathyroid gland
pituitary gland both lobes
rectum mucosa
renal cortex
renal medulla
spleen
stomach
testis
thyroid gland
urinary bladder muscular wall

Cancer tissues

Strong positiveWeak to moderateNo staining at all
esophagus  squamous cell carcinomamuscle-invasive urothelial carcinomabreast cancer no special type
non-invasive urothelial carcinoma (low grade, pTaG2)clear cell ovarian carcinoma
oral squamous cell carcinomaclear cell renal cell carcinoma
pharynx squamous cell carcinomacolorectal adenocarcinoma
endometrioid ovarian carcinoma
esophagus adenocarcinoma
follicular thyroid carcinoma
high-grade serous carcinoma
invasive lobular carcinoma
papillary renal cell carcinoma
prostate adenocarcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant region within aa 600-680 of human TP53 protein (exact sequence is proprietary).

Epitope

Region within aa 600-680

Cellular localization

nucleus

Positive Controls

epididymis, esophagus, esophagus squamous cell carcinoma, pharynx squamous cell carcinoma

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