Description
PD-L1
All names and symbols: B7 homolog 1; CD_antigen: CD274; Programmed cell death 1 ligand 1; Programmed death ligand 1; B7-H; B7H1; B7-H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1; PDCD1 ligand 1
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG, kappa |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 90 |
| RRID | AB_2924905 |
Immunohistochemistry (IHC):
AE00385 to PD-L1 was successfully used to stain plasma membranes in sections of colorectal adenocarcinoma, anaplastic large cell lymphoma, and of follicular thyroid carcinoma. Recommended concentration: 1-3 μg/ml
Formaldehyde-fixed, paraffin-embedded human colorectal adenocarcinoma stained with PD-L1 Rabbit Recombinant Antibody AE00385 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human anaplastic large cell lymphoma stained with PD-L1 Rabbit Recombinant Antibody AE00385 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human follicular thyroid carcinoma stained with PD-L1 Rabbit Recombinant Antibody AE00385 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| lung | appendix mucosa | adipose |
| ovary corpus luteum | duodenum mucosa | adrenal gland |
| placenta early | ileum mucosa | aorta media |
| placenta mature | lymph node | appendix muscular wall |
| thymus | pituitary gland anterior lobe | bone marrow |
| pituitary gland posterior lobe | breast | |
| spleen | bronchus mucosa | |
| tonsil | cerebellum | |
| cerebrum | ||
| colon mucosa | ||
| colon muscular wall | ||
| duodenum Brunner gland | ||
| ectocervix | ||
| endocervix | ||
| endometrium, proliferation | ||
| endometrium, secretion | ||
| epididymis | ||
| esophagus squamous epithelium | ||
| fallopian tube | ||
| gallbladder | ||
| heart muscle | ||
| liver | ||
| myometrium | ||
| ovarian stroma | ||
| pancreas | ||
| parathyroid gland | ||
| parotid gland | ||
| prostate | ||
| rectum mucosa | ||
| renal cortex | ||
| renal medulla | ||
| seminal vesicle | ||
| sinus paranalis | ||
| skin | ||
| stomach | ||
| testis | ||
| thyroid gland | ||
| urinary bladder muscular wall | ||
| urothelium |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| Tumor cells: | clear cell renal cell carcinoma | |
| anaplastic large cell lymphoma | non-invasive papillary urothelial carcinoma | |
| colorectal adenocarcinoma | vulva squamous cell carcinoma | |
| follicular thyroid carcinoma | ||
| gastrointestinal stromal tumor | ||
| prostate adenocarcinoma | ||
| Tumor-associated immune cells: | ||
| basal cell carcinoma | ||
| breast cancer no special type | ||
| mucinous ovarian carcinoma | ||
| urothelial carcinoma invasive |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
Reviews
There are no reviews yet.