PD-L1 Recombinant Antibody AE00385

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE711R to human PD-L1

See all our PD-L1 antibodies

Official Symbol: CD274

GeneID: 29126

Swiss-Prot: Q9NZQ7

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

PD-L1

All names and symbols: B7 homolog 1; CD_antigen: CD274; Programmed cell death 1 ligand 1; Programmed death ligand 1; B7-H; B7H1; B7-H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1; PDCD1 ligand 1

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2924905

Immunohistochemistry (IHC):

AE00385 to PD-L1 was successfully used to stain plasma membranes in sections of colorectal adenocarcinoma, anaplastic large cell lymphoma, and of follicular thyroid carcinoma. Recommended concentration: 1-3 μg/ml

IHC of human colorectal adenocarcinoma stained with PD-L1 Rabbit Recombinant Antibody AE00385
Human colorectal adenocarcinoma with AE00385

Formaldehyde-fixed, paraffin-embedded human colorectal adenocarcinoma stained with PD-L1 Rabbit Recombinant Antibody AE00385 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human large cell lymphoma stained with PD-L1 Rabbit Recombinant Antibody AE00385
Human large cell lymphoma with AE00385

Formaldehyde-fixed, paraffin-embedded human anaplastic large cell lymphoma stained with PD-L1 Rabbit Recombinant Antibody AE00385 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human follicular thyroid carcinoma stained with PD-L1 Rabbit Recombinant Antibody AE00385
Human follicular thyroid carcinoma with AE00384

Formaldehyde-fixed, paraffin-embedded human follicular thyroid carcinoma stained with PD-L1 Rabbit Recombinant Antibody AE00385 at 1-2 mg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
lungappendix mucosaadipose
ovary corpus luteumduodenum mucosaadrenal gland
placenta earlyileum mucosaaorta media
placenta maturelymph nodeappendix muscular wall
thymus pituitary gland anterior lobebone marrow
pituitary gland posterior lobebreast
spleenbronchus mucosa
tonsilcerebellum
cerebrum
colon mucosa
colon muscular wall
duodenum Brunner gland
ectocervix
endocervix
endometrium, proliferation
endometrium, secretion
epididymis
esophagus squamous epithelium
fallopian tube
gallbladder
heart muscle
liver
myometrium
ovarian stroma
pancreas
parathyroid gland
parotid gland
prostate
rectum mucosa
renal cortex
renal medulla
seminal vesicle
sinus paranalis
skin
stomach
testis
thyroid gland
urinary bladder muscular wall
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
Tumor cells:clear cell renal cell carcinoma
anaplastic large cell lymphomanon-invasive papillary urothelial carcinoma
colorectal adenocarcinomavulva squamous cell carcinoma
follicular thyroid carcinoma
gastrointestinal stromal tumor
prostate adenocarcinoma
Tumor-associated immune cells:
basal cell carcinoma
breast cancer no special type
mucinous ovarian carcinoma
urothelial carcinoma invasive
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

KLH-conjugated peptide, its sequence is proprietary

Epitope

Unknown

Cellular localization

plasma membrane

Positive Controls

anaplastic large cell lymphoma, colorectal adenocarcinoma, follicular thyroid carcinoma

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